FastQCFastQC Report
Fri 28 Aug 2020
Bol_2_Feb_20_1_subset_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBol_2_Feb_20_1_subset_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATG197331.9733No Hit
GGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAG162251.6225No Hit
GGACAGGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT93010.9301No Hit
GTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTT74730.7473No Hit
GCTGAACTTAAGCATATCAGTAAGCGGAGGAAAAGAAACTAACGAGGATT74170.7417No Hit
GCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGG66180.6617999999999999No Hit
GGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATAGCCGTCACCT63750.6375No Hit
GCAGAATTCGCCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAG63720.6372No Hit
CTTAGATGTCCGGGGCCGCACATGCGCTACAATGAACGGTGCAACGTGCT58530.5852999999999999No Hit
ACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCG58090.5809No Hit
CGAACGTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCGTCCCGCCT57860.5786No Hit
CGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGAT52280.5228No Hit
TTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT44730.44730000000000003No Hit
GGAAAATCTAGAGCTAATACATGCGAGCAAGCTCCGACCTTCGGGGAAGA42720.42719999999999997No Hit
CGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCGC40350.4035No Hit
GCGAAGCAGAATTCGCCAAGCGTTGGATTGTTCACCCACTAATAGGGAAC39730.3973No Hit
GTCAACTTAGAACTGGTACGGACCAGGGGAATCCGACTGTCTAATTAAAA37460.37460000000000004No Hit
TTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCGTCCCGCCTGAATAA36590.3659No Hit
AGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCG34900.349No Hit
TGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTA34770.3477No Hit
CTGTGATGCCCTTAGATGTCCGGGGCCGCACATGCGCTACAATGAACGGT34210.3421No Hit
GGGACAGGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGA33510.33509999999999995No Hit
GCCGCACATGCGCTACAATGAACGGTGCAACGTGCTTTTTGCCTTGCCCG33050.3305No Hit
GTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCGTCCCGCCTGAATA32840.3284No Hit
CTGAACTTAAGCATATCAGTAAGCGGAGGAAAAGAAACTAACGAGGATTC32070.32070000000000004No Hit
GACAGATTGAGAGCTCTTTCTTGATTCGGTGAGTGGTGGTGCATGGCCGT30480.3048No Hit
CGAGACTCTAGCCTGCTAAATAGTTCGGGGCGCTCCCCTAAACGGGAGCC29630.2963No Hit
GCAACTTCTTAGAGGGACAGGTGGCGTTTAGCCACACGAGATTGAGCAAT28050.2805No Hit
GTCTGTGATGCCCTTAGATGTCCGGGGCCGCACATGCGCTACAATGAACG27920.2792No Hit
GTTAATATTCCCGAACCTGGACACGGAGAGCGACCCGCGAGGGTCCAGTG27330.27330000000000004No Hit
CTCGTCGCAACTTCTTAGAGGGACAGGTGGCGTTTAGCCACACGAGATTG26350.2635No Hit
GCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGA26310.2631No Hit
CAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGGTGAGTGGTGGTGC25990.2599No Hit
GCAAGCTCCGACCTTCGGGGAAGAGCGCATTTGTTAGTACAAAACCAGCC25790.2579No Hit
GTCGCAACTTCTTAGAGGGACAGGTGGCGTTTAGCCACACGAGATTGAGC25320.2532No Hit
GACAGGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATG24130.24130000000000001No Hit
CGACTGTCTAATTAAAACAAAGCATTGCGATAGCCGTCACCTGGTGTTGA23940.23939999999999997No Hit
GTCGGCTCTTCCTATCATTGCGAAGCAGAATTCGCCAAGCGTTGGATTGT23390.2339No Hit
CTCGGTTCTAGTTTCGTTGGTTTGCGAATCGTTGAGGTAATGATTAACAG22870.2287No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTCAATTCCGATAACGAAC22750.2275No Hit
GCGATTTGTCTGGTCAATTCCGATAACGAACGAGACTCTAGCCTGCTAAA22550.2255No Hit
GCGCGAGTCACGGGGCTCGTACGAAACCCAAGGGCGCAGCGAAAGCGAGG22490.22490000000000002No Hit
GGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATAGCCGTCACCTG22390.22390000000000002No Hit
AGGGAATCGGGTTAATATTCCCGAACCTGGACACGGAGAGCGACCCGCGA22320.2232No Hit
GCGGTCCCGAACGTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCGT22110.2211No Hit
CGAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAATAGCCCAGCACCGA21780.2178No Hit
GCCGATTGGCCGGGCGGCGAGCGTCCAAGTCCTCCTGATCGTGGCCCTGC21360.21359999999999998No Hit
GGGAAACCAAAGTGTTTGGGTTCCGGGGGGAGTATGGTTGCAAAACTGAA21220.21220000000000003No Hit
CGGGGCGCTCCCCTAAACGGGAGCCCTCGTCGCAACTTCTTAGAGGGACA20450.2045No Hit
GCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCGCACATGCGCT20440.20439999999999997No Hit
GGTTAATATTCCCGAACCTGGACACGGAGAGCGACCCGCGAGGGTCCAGT18920.1892No Hit
GTTCGGGGCGCTCCCCTAAACGGGAGCCCTCGTCGCAACTTCTTAGAGGG18760.18760000000000002No Hit
CTCAAAGCAGGCGTCCCGCCTGAATAATCCCGCATGGAATAATGGAAGAG18750.1875No Hit
GTTAGTTTTACCCTACTGTTGCCCCTTCGTGTCGTTGCAATGGTAATCTT18490.1849No Hit
GTGATGCCCTTAGATGTCCGGGGCCGCACATGCGCTACAATGAACGGTGC18260.18259999999999998No Hit
GGTCAACTTAGAACTGGTACGGACCAGGGGAATCCGACTGTCTAATTAAA18150.1815No Hit
GTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGTTGCCCCTTCG17460.1746No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCGCACATGCG17260.1726No Hit
GCAAAACTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGA17100.17099999999999999No Hit
GTCCGGGGCCGCACATGCGCTACAATGAACGGTGCAACGTGCTTTTTGCC16840.1684No Hit
CTGGTACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCG16570.16570000000000001No Hit
GTACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATA16450.1645No Hit
CAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCGCACATGCGCTA16230.1623No Hit
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCGCACATGCGCTACAAT16170.16169999999999998No Hit
GAACGTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCGTCCCGCCTG16100.161No Hit
CCTCACCCGGTCTGGACACTGCAAGGATTGACAGATTGAGAGCTCTTTCT16040.1604No Hit
GGGCGCTCCCCTAAACGGGAGCCCTCGTCGCAACTTCTTAGAGGGACAGG16030.1603No Hit
GTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGT15740.1574No Hit
GGCGCATCGAATGCGAAGTGTCAAGTGGGCCACTTTTGGTAAGCAGAACT15630.1563No Hit
CTTAGAACTGGTACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAG15420.1542No Hit
GTCCGGTTAAGGCGCCCGATGCTGACGCTCATCAGATACCATAAAAGGTG14630.1463No Hit
GTTTTACCCTACTGTTGCCCCTTCGTGTCGTTGCAATGGTAATCTTGCTC14580.1458No Hit
GCTCGCTCAAGTTCGGGTTTCGGCTCGCGCTGGAGCGGCGCATCGAATGC14520.1452No Hit
GGGAATCGGGTTAATATTCCCGAACCTGGACACGGAGAGCGACCCGCGAG13930.13929999999999998No Hit
AAAAAATTAGAGTGCTCAAAGCAGGCGTCCCGCCTGAATAATCCCGCATG13930.13929999999999998No Hit
GATGCCCTTAGATGTCCGGGGCCGCACATGCGCTACAATGAACGGTGCAA13770.1377No Hit
GGGGCGCTCCCCTAAACGGGAGCCCTCGTCGCAACTTCTTAGAGGGACAG13650.13649999999999998No Hit
ATTGAGAGCTCTTTCTTGATTCGGTGAGTGGTGGTGCATGGCCGTTCTTA13440.1344No Hit
CGAGATTACCCGCTGAACTTAAGCATATCAGTAAGCGGAGGAAAAGAAAC13260.1326No Hit
GCCGAATCAACTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGAGCCTAT13150.1315No Hit
GTCAGAAGCGCCGCTCGCTCAAGTTCGGGTTTCGGCTCGCGCTGGAGCGG13140.13140000000000002No Hit
GCCGATCGCACGGCCCCGCGCCGGCGACGCATCTTTCAAATGTCTGCCCT12400.124No Hit
GGCGAGTGAAGCGGGAATAGCCCAGCACCGAATCCCCCGGCTCGAGGTCG12300.123No Hit
GAGCAAGCTCCGACCTTCGGGGAAGAGCGCATTTGTTAGTACAAAACCAG12290.12290000000000001No Hit
CGCACATGCGCTACAATGAACGGTGCAACGTGCTTTTTGCCTTGCCCGAG12260.12260000000000001No Hit
ATCGGGTTAATATTCCCGAACCTGGACACGGAGAGCGACCCGCGAGGGTC12160.12160000000000001No Hit
GGGAAATGTGGTGTATAGGAAGCCGATTGGCCGGGCGGCGAGCGTCCAAG11890.11889999999999999No Hit
GGAAGAGGACCTCGGTTCTAGTTTCGTTGGTTTGCGAATCGTTGAGGTAA11850.11850000000000001No Hit
GCTAGGCCCGTGAGGCGCGCCAGCCGCTTCTGCCTTCGAGCTTCCTCGGA11600.116No Hit
CAAGCTCCGACCTTCGGGGAAGAGCGCATTTGTTAGTACAAAACCAGCCC11560.1156No Hit
GCCGCTCGCTCAAGTTCGGGTTTCGGCTCGCGCTGGAGCGGCGCATCGAA11190.1119No Hit
GGCGTCCCGCCTGAATAATCCCGCATGGAATAATGGAAGAGGACCTCGGT11190.1119No Hit
GCGAAGTGTCAAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGA11150.11150000000000002No Hit
AGAACTGGTACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCAT11040.1104No Hit
GGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGA11010.1101No Hit
CGATAACGAACGAGACTCTAGCCTGCTAAATAGTTCGGGGCGCTCCCCTA10860.10859999999999999No Hit
GTCTAATTAAAACAAAGCATTGCGATAGCCGTCACCTGGTGTTGACGCAA10820.1082No Hit
GCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGCGAA10820.1082No Hit
ATTCGCCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGG10580.10579999999999999No Hit
CTTTGAAAAAATTAGAGTGCTCAAAGCAGGCGTCCCGCCTGAATAATCCC10560.10560000000000001No Hit
CGGCAAGTCAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGACCGG10320.10319999999999999No Hit
GGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCT10060.1006No Hit
GAAAATCTAGAGCTAATACATGCGAGCAAGCTCCGACCTTCGGGGAAGAG10030.1003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)