FastQCFastQC Report
Fri 28 Aug 2020
Bol_2_Feb_20_1_subset_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBol_2_Feb_20_1_subset_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACT298712.9871000000000003No Hit
CAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGG219532.1953No Hit
GGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATT182901.8290000000000002No Hit
GGCGGATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTG176291.7629No Hit
CAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCT130601.306No Hit
CCGCATTCTGGGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCC110691.1069No Hit
GGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTC105101.051No Hit
ACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGG104171.0416999999999998No Hit
GCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCC100671.0067No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGC85910.8591No Hit
GACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTCGTT76840.7684No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCT76310.7631No Hit
CGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTGATCGTCT71400.714No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT60310.6031No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGC57920.5792No Hit
GCCGCATTCTGGGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCC56000.5599999999999999No Hit
ATTTAGCCTTAGATGGAGTTTACCACCCACCTTTGGGCTGCATTCCCAAA52300.523No Hit
GGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTCG50230.5023No Hit
GTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTA49610.4961No Hit
AGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAAT49320.49319999999999997No Hit
GCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGGATGGTAGCT41690.4169No Hit
AAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGA40670.4067No Hit
GCATTCTGGGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGG36790.3679No Hit
GTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCTCCCTTCAAAGTTC35950.3595No Hit
CGGATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTGAT35710.3571No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCG35680.3568No Hit
GGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGGATGGTAGCTTC34370.3437No Hit
CCGTATTTAGCCTTAGATGGAGTTTACCACCCACCTTTGGGCTGCATTCC33500.335No Hit
ATTTTTCGTCACTACCTCGCCGTGTCGGCATTGGGTAATTTGCGTGCCTG32810.3281No Hit
CGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGGATGGTAGCTT31900.319No Hit
GGCGGTTCTTCCTCGGCCCCGACGGCGCGTCGGCCACGGCGGGCTGTAAC31840.3184No Hit
CTCCCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTCCGCTATC31600.316No Hit
ATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGG31070.3107No Hit
GAACTATTTAGCAGGCTAGAGTCTCGTTCGTTATCGGAATTGACCAGACA30930.30929999999999996No Hit
GGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTCGT30750.3075No Hit
GCGGATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTGA30630.3063No Hit
GTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTCGTTCAT29350.2935No Hit
GGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGGATGGTAGC29270.2927No Hit
GGCGACGGTCCGGTATAGGCTCGACGCTCCAGCGCCATCCATTTTCAGGG28290.2829No Hit
GCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGC28250.2825No Hit
GGCAAGTTGTGCGGCGACGGTCCGGTATAGGCTCGACGCTCCAGCGCCAT27640.2764No Hit
CTCCGCTCCACTGAGTAAGTAAAGAAACTACAAAAGTAGTGGTATTTCAA27320.2732No Hit
ATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTGATCGT27200.272No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACT25740.25739999999999996No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA25720.2572No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTC25450.2545No Hit
CTCCTATCGCTTAGGGCCGACTGACCCATGTTCAACTGCTGTTCACATGG24170.2417No Hit
CTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCG23730.2373No Hit
CGTGGCTCTGCCACCGCGCCGGGACGGGATGACCCGCCCGTTCGCGCGCG22950.2295No Hit
GTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCAT22510.2251No Hit
AAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAA21900.219No Hit
GTTCTTTTCAACTTTCCCTCACGGTACTTGTCCGCTATCGGACTCGTGCC21360.21359999999999998No Hit
GTCCACCCGTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCTCCCTT21350.21350000000000002No Hit
GCAAGTTGTGCGGCGACGGTCCGGTATAGGCTCGACGCTCCAGCGCCATC21170.2117No Hit
GTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCA21120.21120000000000003No Hit
GCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACA20920.2092No Hit
GCGACGGTCCGGTATAGGCTCGACGCTCCAGCGCCATCCATTTTCAGGGC20750.2075No Hit
CGACGGTCCGGTATAGGCTCGACGCTCCAGCGCCATCCATTTTCAGGGCT20580.20579999999999998No Hit
GGATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTGATC20120.2012No Hit
CGAGAATCACACGGTCTCCCTCGGATTTTCAAGGGCCGGCGGGGGCGCTC20100.201No Hit
CTTGAACTCTCCCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT19650.1965No Hit
CGCATTCTGGGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCG19420.19419999999999998No Hit
GATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTGATCG19010.1901No Hit
CCGCCCTCCGCTCCACTGAGTAAGTAAAGAAACTACAAAAGTAGTGGTAT18860.18860000000000002No Hit
GGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTC18520.1852No Hit
AGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTC18230.1823No Hit
GCGGTTCTTCCTCGGCCCCGACGGCGCGTCGGCCACGGCGGGCTGTAACT18060.1806No Hit
GGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCAATCCCTGGTACG18030.1803No Hit
GTACTCTTGAACTCTCCCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA17560.1756No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTCCGCTATCGGAC17510.1751No Hit
CGGGACTCATCCATATCGCTCATTAACTGGAACAATCGGTCGTAGCGACG17400.174No Hit
ACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTCGTTC16970.1697No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT16810.1681No Hit
GTAGCTTCGCACCATTGGCTTATCAGCCAAGCACATATACAAAATGTCCG16690.1669No Hit
GCGGTTTCACGTACTCTTGAACTCTCCCTTCAAAGTTCTTTTCAACTTTC16350.1635No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC16180.1618No Hit
GTTATTTTTCGTCACTACCTCGCCGTGTCGGCATTGGGTAATTTGCGTGC16170.16169999999999998No Hit
GCTGGTGCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAG16160.1616No Hit
GCGGGTCCACCCGTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCTC15720.1572No Hit
GTGCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAG15410.15410000000000001No Hit
CGGGTCCACCCGTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCTCC14960.14959999999999998No Hit
GTTTAGGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACT14550.1455No Hit
GCGGATTTCCCGGCCCTCTCGGGCAAGGCAAAAAGCACGTTGCACCGTTC14290.1429No Hit
GTCCGGTATAGGCTCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTG14070.1407No Hit
GGGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGGATGGTAG13910.1391No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT13610.1361No Hit
GGCAACAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCT13580.1358No Hit
CGTATTTAGCCTTAGATGGAGTTTACCACCCACCTTTGGGCTGCATTCCC13550.1355No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG13550.1355No Hit
ATTCGCTTTACCGGATCAAACTGCTCTCCGAGTGTCAGCTATCCTGAGGG13460.1346No Hit
CTTAGATGGAGTTTACCACCCACCTTTGGGCTGCATTCCCAAACAACCCG13390.1339No Hit
GCAGAAATCACATTGCGTCAACACCAGGTGACGGCTATCGCAATGCTTTG13390.1339No Hit
ATTTTACACCGCTGCGTTGCATCCGGTGGACTCGCACCGAGGGGCCAGCG13380.1338No Hit
GCAGGCTAGAGTCTCGTTCGTTATCGGAATTGACCAGACAAATCGCTCCA12930.1293No Hit
CGGCGGATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCT12750.1275No Hit
CCGGGACTCATCCATATCGCTCATTAACTGGAACAATCGGTCGTAGCGAC12620.1262No Hit
CACCCGTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCTCCCTTCAA12410.12409999999999999No Hit
GGTCCACCCGTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCTCCCT12370.1237No Hit
GCTGGCACCAGACTTGCCCTCCAATAGATCCTCGTTAAAGGATTTAGAGT12360.12359999999999999No Hit
TGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCA12350.1235No Hit
TGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTT11980.1198No Hit
CAGAAATCACATTGCGTCAACACCAGGTGACGGCTATCGCAATGCTTTGT11830.1183No Hit
CTGGGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGGATGGT11770.11770000000000001No Hit
TTTAGCCTTAGATGGAGTTTACCACCCACCTTTGGGCTGCATTCCCAAAC11680.1168No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC11630.1163No Hit
CCCGTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCTCCCTTCAAAG11430.11429999999999998No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATAGATCCTCGTTAAAGGATT11310.1131No Hit
ATTCTGGGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGGAT11290.1129No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG11270.1127No Hit
GGTAGCTTCGCACCATTGGCTTATCAGCCAAGCACATATACAAAATGTCC11220.1122No Hit
ATTTAGCAGGCTAGAGTCTCGTTCGTTATCGGAATTGACCAGACAAATCG11210.1121No Hit
CTTCGCACCATTGGCTTATCAGCCAAGCACATATACAAAATGTCCGAACC11100.11100000000000002No Hit
GTACGAACGGGGTTCAGCGGATTTCCCGGCCCTCTCGGGCAAGGCAAAAA10940.1094No Hit
GTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTGATCGTCTTCGAA10800.108No Hit
GTTCTTCCTCGGCCCCGACGGCGCGTCGGCCACGGCGGGCTGTAACTCTC10650.1065No Hit
ATCGCTTAGGGCCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCC10550.1055No Hit
TGCGGGTCCACCCGTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCT10530.10529999999999999No Hit
CCGGCGGATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATC10490.10490000000000001No Hit
AATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTCGTTCATGG10490.10490000000000001No Hit
CCCGTATTTAGCCTTAGATGGAGTTTACCACCCACCTTTGGGCTGCATTC10480.1048No Hit
TTAGCCTTAGATGGAGTTTACCACCCACCTTTGGGCTGCATTCCCAAACA10460.1046No Hit
CGGCGATCCACGAATTTCACCTCTCCCGCCGCAATACGTATGCCCCCGGC10350.1035No Hit
CGGCGCTTCTGACCCATTTAAAGTTTGAGAATAGGTTAAGGTCACTCGAA10290.10289999999999999No Hit
CCAAGATCTGCACTCGGGGCGGCTCCACCCGCGCTCGCGCGCCAGGCTTC10250.10250000000000001No Hit
CTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC10160.1016No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)