FastQCFastQC Report
Fri 28 Aug 2020
Bol_2_Feb_20_1_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBol_2_Feb_20_1_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66102967
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATG12058131.824143536552603No Hit
GGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAG9877271.4942249112660857No Hit
GGACAGGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT5707580.8634377939495514No Hit
GTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTT4542000.687109853934393No Hit
GCTGAACTTAAGCATATCAGTAAGCGGAGGAAAAGAAACTAACGAGGATT4387390.6637205860971415No Hit
GCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGG4124560.6239598897277939No Hit
GGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATAGCCGTCACCT3866360.5848996157767019No Hit
GCAGAATTCGCCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAG3822430.5782539231559757No Hit
CGAACGTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCGTCCCGCCT3620850.5477590741123617No Hit
ACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCG3585110.5423523576483337No Hit
CTTAGATGTCCGGGGCCGCACATGCGCTACAATGAACGGTGCAACGTGCT3561220.5387382989934476No Hit
CGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGAT3228880.48846218960186766No Hit
TTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT2679080.4052889184232835No Hit
CGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCGC2517710.380877003599551No Hit
GGAAAATCTAGAGCTAATACATGCGAGCAAGCTCCGACCTTCGGGGAAGA2503060.3786607641983756No Hit
GCGAAGCAGAATTCGCCAAGCGTTGGATTGTTCACCCACTAATAGGGAAC2363640.3575694264979059No Hit
GTCAACTTAGAACTGGTACGGACCAGGGGAATCCGACTGTCTAATTAAAA2347010.35505365439950676No Hit
TTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCGTCCCGCCTGAATAA2263120.3423628473439021No Hit
TGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTA2092040.31648201207065335No Hit
GTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCGTCCCGCCTGAATA2066430.3126077532949466No Hit
GGGACAGGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGA2048580.3099074206457329No Hit
CTGTGATGCCCTTAGATGTCCGGGGCCGCACATGCGCTACAATGAACGGT2030890.3072312926589211No Hit
AGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCG2022590.3059756757968216No Hit
GCCGCACATGCGCTACAATGAACGGTGCAACGTGCTTTTTGCCTTGCCCG1966190.2974435323001462No Hit
CTGAACTTAAGCATATCAGTAAGCGGAGGAAAAGAAACTAACGAGGATTC1920940.2905981512146043No Hit
CGAGACTCTAGCCTGCTAAATAGTTCGGGGCGCTCCCCTAAACGGGAGCC1811920.2741056993704988No Hit
GACAGATTGAGAGCTCTTTCTTGATTCGGTGAGTGGTGGTGCATGGCCGT1795540.27162774705710263No Hit
GTCTGTGATGCCCTTAGATGTCCGGGGCCGCACATGCGCTACAATGAACG1721720.2604603209414186No Hit
GCAACTTCTTAGAGGGACAGGTGGCGTTTAGCCACACGAGATTGAGCAAT1683320.2546512019649587No Hit
GTTAATATTCCCGAACCTGGACACGGAGAGCGACCCGCGAGGGTCCAGTG1651580.2498496020609786No Hit
CTCGTCGCAACTTCTTAGAGGGACAGGTGGCGTTTAGCCACACGAGATTG1631160.24676048202193404No Hit
GTCGCAACTTCTTAGAGGGACAGGTGGCGTTTAGCCACACGAGATTGAGC1594060.24114802592748977No Hit
GCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGA1593240.24102397703267994No Hit
CAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGGTGAGTGGTGGTGC1558400.2357534117946627No Hit
GACAGGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATG1504760.22763879872442033No Hit
GCAAGCTCCGACCTTCGGGGAAGAGCGCATTTGTTAGTACAAAACCAGCC1497250.22650269238293042No Hit
CGACTGTCTAATTAAAACAAAGCATTGCGATAGCCGTCACCTGGTGTTGA1440590.21793121630985188No Hit
GCGGTCCCGAACGTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCGT1432090.2166453436197501No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTCAATTCCGATAACGAAC1406480.2127710848440434No Hit
AGGGAATCGGGTTAATATTCCCGAACCTGGACACGGAGAGCGACCCGCGA1366350.2067002529553628No Hit
GTCGGCTCTTCCTATCATTGCGAAGCAGAATTCGCCAAGCGTTGGATTGT1356200.20516476968424124No Hit
GCGATTTGTCTGGTCAATTCCGATAACGAACGAGACTCTAGCCTGCTAAA1354240.20486826256981777No Hit
GGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATAGCCGTCACCTG1346280.20366408061532246No Hit
CTCGGTTCTAGTTTCGTTGGTTTGCGAATCGTTGAGGTAATGATTAACAG1324060.20030265812425635No Hit
GCCGATTGGCCGGGCGGCGAGCGTCCAAGTCCTCCTGATCGTGGCCCTGC1295540.19598817705111482No Hit
CGAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAATAGCCCAGCACCGA1283840.19421821111297471No Hit
GCGCGAGTCACGGGGCTCGTACGAAACCCAAGGGCGCAGCGAAAGCGAGG1283340.1941425715429687No Hit
GGGAAACCAAAGTGTTTGGGTTCCGGGGGGAGTATGGTTGCAAAACTGAA1281130.19380824464354227No Hit
GCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCGCACATGCGCT1258120.1903273116318667No Hit
CGGGGCGCTCCCCTAAACGGGAGCCCTCGTCGCAACTTCTTAGAGGGACA1185490.1793399076927969No Hit
GTGATGCCCTTAGATGTCCGGGGCCGCACATGCGCTACAATGAACGGTGC1128250.17068068971851144No Hit
CTCAAAGCAGGCGTCCCGCCTGAATAATCCCGCATGGAATAATGGAAGAG1122550.16981839862044318No Hit
GTTAGTTTTACCCTACTGTTGCCCCTTCGTGTCGTTGCAATGGTAATCTT1121520.16966258110623084No Hit
GGTTAATATTCCCGAACCTGGACACGGAGAGCGACCCGCGAGGGTCCAGT1105380.16722093578643754No Hit
GTTCGGGGCGCTCCCCTAAACGGGAGCCCTCGTCGCAACTTCTTAGAGGG1088040.1645977554986299No Hit
GGTCAACTTAGAACTGGTACGGACCAGGGGAATCCGACTGTCTAATTAAA1082310.16373092602636127No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCGCACATGCG1069360.1617718611632062No Hit
GTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGTTGCCCCTTCG1053260.15933626700901338No Hit
GCAAAACTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGA1028510.15559210829371697No Hit
CTGGTACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCG1002690.15168608089860777No Hit
GTCCGGGGCCGCACATGCGCTACAATGAACGGTGCAACGTGCTTTTTGCC1001820.15155446804679737No Hit
GGGCGCTCCCCTAAACGGGAGCCCTCGTCGCAACTTCTTAGAGGGACAGG984470.14892977496758958No Hit
CCTCACCCGGTCTGGACACTGCAAGGATTGACAGATTGAGAGCTCTTTCT968930.1465788971318035No Hit
GTACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATA965910.14612203412896732No Hit
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCGCACATGCGCTACAAT957700.144880032389469No Hit
GTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGT950050.1437227469683774No Hit
GAACGTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCGTCCCGCCTG933980.14129169118838494No Hit
CAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCGCACATGCGCTA929400.14059883272713009No Hit
GGCGCATCGAATGCGAAGTGTCAAGTGGGCCACTTTTGGTAAGCAGAACT914820.13839318286575547No Hit
GTCCGGTTAAGGCGCCCGATGCTGACGCTCATCAGATACCATAAAAGGTG881000.13327692235055047No Hit
GCTCGCTCAAGTTCGGGTTTCGGCTCGCGCTGGAGCGGCGCATCGAATGC876590.13260978134309764No Hit
CTTAGAACTGGTACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAG865990.13100622245897073No Hit
GCCGAATCAACTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGAGCCTAT852100.1289049552042044No Hit
GGGAATCGGGTTAATATTCCCGAACCTGGACACGGAGAGCGACCCGCGAG848220.1283179921409579No Hit
GATGCCCTTAGATGTCCGGGGCCGCACATGCGCTACAATGAACGGTGCAA846580.12806989435133828No Hit
GTTTTACCCTACTGTTGCCCCTTCGTGTCGTTGCAATGGTAATCTTGCTC843620.12762210809690283No Hit
GTCAGAAGCGCCGCTCGCTCAAGTTCGGGTTTCGGCTCGCGCTGGAGCGG829910.12554807108733865No Hit
AAAAAATTAGAGTGCTCAAAGCAGGCGTCCCGCCTGAATAATCCCGCATG825620.12489908357668725No Hit
CGAGATTACCCGCTGAACTTAAGCATATCAGTAAGCGGAGGAAAAGAAAC817330.12364497950598799No Hit
GGGGCGCTCCCCTAAACGGGAGCCCTCGTCGCAACTTCTTAGAGGGACAG804200.12165868439763074No Hit
ATTGAGAGCTCTTTCTTGATTCGGTGAGTGGTGGTGCATGGCCGTTCTTA771410.11669824139663806No Hit
CGCACATGCGCTACAATGAACGGTGCAACGTGCTTTTTGCCTTGCCCGAG747730.11311595136115449No Hit
GCCGATCGCACGGCCCCGCGCCGGCGACGCATCTTTCAAATGTCTGCCCT744150.11257437203991161No Hit
GGAAGAGGACCTCGGTTCTAGTTTCGTTGGTTTGCGAATCGTTGAGGTAA734360.11109334925919437No Hit
ATCGGGTTAATATTCCCGAACCTGGACACGGAGAGCGACCCGCGAGGGTC729770.11039897800653942No Hit
GGCGAGTGAAGCGGGAATAGCCCAGCACCGAATCCCCCGGCTCGAGGTCG724970.10967283813448192No Hit
GGGAAATGTGGTGTATAGGAAGCCGATTGGCCGGGCGGCGAGCGTCCAAG724710.10963350555807881No Hit
GCTAGGCCCGTGAGGCGCGCCAGCCGCTTCTGCCTTCGAGCTTCCTCGGA719910.10890736568602133No Hit
GAGCAAGCTCCGACCTTCGGGGAAGAGCGCATTTGTTAGTACAAAACCAG712710.1078181558779351No Hit
CAAGCTCCGACCTTCGGGGAAGAGCGCATTTGTTAGTACAAAACCAGCCC710500.10748382897850864No Hit
GCGAAGTGTCAAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGA708030.10711016950267906No Hit
GGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGA691510.10461103810968121No Hit
CGATAACGAACGAGACTCTAGCCTGCTAAATAGTTCGGGGCGCTCCCCTA684450.10354300738119666No Hit
GGCGTCCCGCCTGAATAATCCCGCATGGAATAATGGAAGAGGACCTCGGT676280.10230705680729882No Hit
GTCTAATTAAAACAAAGCATTGCGATAGCCGTCACCTGGTGTTGACGCAA666330.10080182936417967No Hit
CTTTGAAAAAATTAGAGTGCTCAAAGCAGGCGTCCCGCCTGAATAATCCC662380.10020427676113237No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)