FastQCFastQC Report
Fri 28 Aug 2020
Bol_2_Feb_20_1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBol_2_Feb_20_1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66102967
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACT18982602.871671403191327No Hit
CAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGG13833182.09267157403086No Hit
GGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATT11274501.7055966640650184No Hit
GGCGGATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTG11191591.6930541105666255No Hit
CAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCT8268071.2507865191588148No Hit
CCGCATTCTGGGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCC6987641.057084169913281No Hit
GGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTC6794801.0279115005533717No Hit
ACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGG6663911.008110573917204No Hit
GCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCC6355380.9614364208493092No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGC5361860.8111375696646113No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCT4876320.7376854960231967No Hit
GACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTCGTT4864260.7358610695946523No Hit
CGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTGATCGTCT4487560.6788742175521411No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGC3744630.5664844060630441No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT3742720.5661954629056212No Hit
GCCGCATTCTGGGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCC3489250.5278507392867857No Hit
GGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTCG3216720.486622635259322No Hit
ATTTAGCCTTAGATGGAGTTTACCACCCACCTTTGGGCTGCATTCCCAAA3215440.4864289979601067No Hit
AGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAAT3196460.4835577198826794No Hit
GTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTA3091850.4677324090460266No Hit
GCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGGATGGTAGCT2639720.39933457147241214No Hit
AAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGA2625820.3972317914262457No Hit
CGGATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTGAT2343270.354487870415862No Hit
GCATTCTGGGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGG2316040.3503685394333359No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCG2288530.3462068502916064No Hit
GTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCTCCCTTCAAAGTTC2182130.3301107497943322No Hit
GGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGGATGGTAGCTTC2159760.3267266354322643No Hit
CCGTATTTAGCCTTAGATGGAGTTTACCACCCACCTTTGGGCTGCATTCC2128330.32197193206168795No Hit
ATTTTTCGTCACTACCTCGCCGTGTCGGCATTGGGTAATTTGCGTGCCTG2105060.3184516664736093No Hit
GGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTCGT2006810.3035884909674327No Hit
GGCGGTTCTTCCTCGGCCCCGACGGCGCGTCGGCCACGGCGGGCTGTAAC1992370.30140402018565976No Hit
CTCCCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTCCGCTATC1990160.3010696932862333No Hit
ATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGG1984740.30024976034736833No Hit
GAACTATTTAGCAGGCTAGAGTCTCGTTCGTTATCGGAATTGACCAGACA1983760.3001015067901566No Hit
CGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGGATGGTAGCTT1940760.2935965037696417No Hit
GCGGATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTGA1905060.28819583847121416No Hit
GTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTCGTTCAT1850040.2798724601877553No Hit
GGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGGATGGTAGC1806240.27324643385523073No Hit
GGCGACGGTCCGGTATAGGCTCGACGCTCCAGCGCCATCCATTTTCAGGG1781850.26955673563033866No Hit
GGCAAGTTGTGCGGCGACGGTCCGGTATAGGCTCGACGCTCCAGCGCCAT1773740.26832986180484153No Hit
CTCCGCTCCACTGAGTAAGTAAAGAAACTACAAAAGTAGTGGTATTTCAA1770470.2678351790170024No Hit
GCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGC1747900.2644208088269321No Hit
ATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTGATCGT1722470.2605737802964275No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTC1619260.24496026025579154No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACT1614220.2441978133901312No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA1588700.24033716973702557No Hit
CTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCG1531830.23173392504454451No Hit
CTCCTATCGCTTAGGGCCGACTGACCCATGTTCAACTGCTGTTCACATGG1488050.2251109242948202No Hit
AAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAA1483340.2243983995453638No Hit
CGTGGCTCTGCCACCGCGCCGGGACGGGATGACCCGCCCGTTCGCGCGCG1415330.21410990523314938No Hit
GTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCAT1404310.21244280911021737No Hit
GTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCA1334940.20194857516758666No Hit
GTCCACCCGTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCTCCCTT1332740.2016157610595603No Hit
GTTCTTTTCAACTTTCCCTCACGGTACTTGTCCGCTATCGGACTCGTGCC1322720.20009994407664033No Hit
CGACGGTCCGGTATAGGCTCGACGCTCCAGCGCCATCCATTTTCAGGGCT1300500.1967385215855742No Hit
GCAAGTTGTGCGGCGACGGTCCGGTATAGGCTCGACGCTCCAGCGCCATC1289380.19505629754864104No Hit
GCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACA1285660.1944935391477965No Hit
GCGACGGTCCGGTATAGGCTCGACGCTCCAGCGCCATCCATTTTCAGGGC1277290.19322733274589626No Hit
GGATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTGATC1261210.1907947641745037No Hit
CGAGAATCACACGGTCTCCCTCGGATTTTCAAGGGCCGGCGGGGGCGCTC1258290.19035302908566873No Hit
CGCATTCTGGGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCG1222780.18498110682384347No Hit
CTTGAACTCTCCCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT1205680.18239423352963868No Hit
GATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTGATCG1180260.17854871779053427No Hit
AGGGACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTC1162180.17581359093911775No Hit
CCGCCCTCCGCTCCACTGAGTAAGTAAAGAAACTACAAAAGTAGTGGTAT1151180.174149520398986No Hit
GCGGTTCTTCCTCGGCCCCGACGGCGCGTCGGCCACGGCGGGCTGTAACT1136510.17193025541501034No Hit
GGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTC1136260.17189243563000736No Hit
GGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCAATCCCTGGTACG1119500.16935699724340664No Hit
CGGGACTCATCCATATCGCTCATTAACTGGAACAATCGGTCGTAGCGACG1089980.16489123703025313No Hit
ACGTAATCAACGCGAGCTAATGACTCGCGCTTACTGGGAATTCCTCGTTC1087780.16455842292222678No Hit
GTACTCTTGAACTCTCCCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA1086720.1643980670338141No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTCCGCTATCGGAC1077560.16301235011130438No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT1048670.1586418957563584No Hit
GTTATTTTTCGTCACTACCTCGCCGTGTCGGCATTGGGTAATTTGCGTGC1040790.15744981613306402No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC1016790.15381911677277663No Hit
GTAGCTTCGCACCATTGGCTTATCAGCCAAGCACATATACAAAATGTCCG1013860.15337586889254154No Hit
GCTGGTGCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAG996580.1507617653531346No Hit
GCGGGTCCACCCGTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCTC977790.14791923031230958No Hit
GTGCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAG973120.14721275672845366No Hit
GCGGTTTCACGTACTCTTGAACTCTCCCTTCAAAGTTCTTTTCAACTTTC969380.14664697274480887No Hit
GTTTAGGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACT921690.13943247055763774No Hit
GGGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGGATGGTAG910960.13780924538530925No Hit
CGGGTCCACCCGTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCTCC899450.13606802248377142No Hit
GCGGATTTCCCGGCCCTCTCGGGCAAGGCAAAAAGCACGTTGCACCGTTC891120.13480786724747168No Hit
CTTAGATGGAGTTTACCACCCACCTTTGGGCTGCATTCCCAAACAACCCG873270.13210753459825791No Hit
ATTCGCTTTACCGGATCAAACTGCTCTCCGAGTGTCAGCTATCCTGAGGG871780.13188212867964005No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT865280.1308988142695622No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG859780.13006677899949634No Hit
GTCCGGTATAGGCTCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTG854820.12931643446503696No Hit
CGTATTTAGCCTTAGATGGAGTTTACCACCCACCTTTGGGCTGCATTCCC837090.12663425531262462No Hit
CGGCGGATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCT835420.1263816191488046No Hit
GGCAACAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCT833420.12607906086878068No Hit
CCGGGACTCATCCATATCGCTCATTAACTGGAACAATCGGTCGTAGCGAC813710.12309734901914463No Hit
GCAGAAATCACATTGCGTCAACACCAGGTGACGGCTATCGCAATGCTTTG807300.12212764973166786No Hit
ATTTTACACCGCTGCGTTGCATCCGGTGGACTCGCACCGAGGGGCCAGCG806430.12199603687985745No Hit
GGTCCACCCGTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCTCCCT782590.11838954218197195No Hit
CACCCGTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCTCCCTTCAA780310.11804462574274464No Hit
GCAGGCTAGAGTCTCGTTCGTTATCGGAATTGACCAGACAAATCGCTCCA776700.11749850804730143No Hit
TGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCA768440.11624894235080249No Hit
TGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTAATGACTCGCGCTT754710.11417187975843808No Hit
GCTGGCACCAGACTTGCCCTCCAATAGATCCTCGTTAAAGGATTTAGAGT751120.11362878764579508No Hit
CTGGGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGGATGGT748820.11328084562376754No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG747040.11301156875454622No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC746450.11292231406193914No Hit
CAGAAATCACATTGCGTCAACACCAGGTGACGGCTATCGCAATGCTTTGT738990.1117937716774498No Hit
GGTAGCTTCGCACCATTGGCTTATCAGCCAAGCACATATACAAAATGTCC738950.11178772051184933No Hit
TTTAGCCTTAGATGGAGTTTACCACCCACCTTTGGGCTGCATTCCCAAAC732180.11076356073396827No Hit
ATTCTGGGCGGGATTCCGACTTAGAGGCGTTCAGCCGTAATCCCCCGGAT711840.10768654302612468No Hit
ATCGCTTAGGGCCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCC703520.10642790058122505No Hit
GTCGGCATCGTTTAGGGTCAGAACTACGACGGTATCTGATCGTCTTCGAA697710.10554896877775546No Hit
GTACGAACGGGGTTCAGCGGATTTCCCGGCCCTCTCGGGCAAGGCAAAAA696330.10534020356453894No Hit
CTTCGCACCATTGGCTTATCAGCCAAGCACATATACAAAATGTCCGAACC695960.10528423028273451No Hit
CCCGTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCTCCCTTCAAAG694850.10511631043732123No Hit
ATTTAGCAGGCTAGAGTCTCGTTCGTTATCGGAATTGACCAGACAAATCG694640.1050845418179187No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATAGATCCTCGTTAAAGGATT692760.10480013703469619No Hit
TGCGGGTCCACCCGTTTACCTCTATGCGGTTTCACGTACTCTTGAACTCT668430.10111951555820482No Hit
CCGGCGGATCGACAGTCGGCATCGTTTAGGGTCAGAACTACGACGGTATC665990.1007503944565756No Hit
CGGCGCTTCTGACCCATTTAAAGTTTGAGAATAGGTTAAGGTCACTCGAA664440.10051591178955703No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)