FastQCFastQC Report
Wed 2 Jan 2019
Hp-R-neg_S26_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-R-neg_S26_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13467731
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG212000.15741330146852503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG24150.031.522896145
AGCCGGT28500.024.416966
GCCGGTA29500.023.5893547
CCGGTAC40650.017.1192828
TACGCTA42350.017.118099
GGGATCT55100.016.0657481
GACGTTA79050.015.4055014
TCCTGGG23200.015.31306145
CCCTACT37050.015.0650067
TCGTGTT74950.014.993878145
AAGCCGG49450.014.9519935
GGACGTT77350.014.8070473
GTCGACT80700.014.4758831
CCAGTCC88300.014.2844478
CTCCCTA35550.014.2732746
CTACCAG64350.014.1937418
GCGTCGG32450.013.852587145
ACGTTAC87650.013.8111485
TTGAGTC60550.013.7673557
CTCGGTT130850.013.6412481
Produced by FastQC (version 0.11.5)