FastQCFastQC Report
Wed 2 Jan 2019
Hp-R-neg_S26_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-R-neg_S26_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13467731
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA139940.10390762928068581No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGGT31000.021.2851146
GCCGGTA33000.018.893887
TAGCCAG34050.018.524249
TACGCTA44400.018.125019
CTACCAG61800.015.9546418
GACTCCA94300.015.60920054
ACCCAAG54100.015.2793166
CCGGTAC46750.014.8876548
CCAGTCC93050.014.8817378
CTCGGTT130900.014.513351
GACGTTA74600.014.4825084
CTCGCCA51150.014.4574879
GGACGTT72450.014.4118713
CTAGCTT41400.014.3598598
CGACTCT31550.014.2494571
GTTAAGG120600.013.6481845
CTACGTT61750.013.61939058
TACCAGG75300.013.47942359
TAACTCG104200.013.2197779
GTCGACT84350.013.1526211
Produced by FastQC (version 0.11.5)