FastQCFastQC Report
Wed 2 Jan 2019
Hp-R-3_S18_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-R-3_S18_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13782675
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT386680.2805551172033005No Hit
CTCGGTTAAGGTGCCTAAGGCTGACGCTCATGAGATACCATAAAAGGTGT286050.20754316560464497No Hit
GGTTAAGGTGCCTAAGGCTGACGCTCATGAGATACCATAAAAGGTGTTGG284930.2067305512173798No Hit
GCCAGTTCTAAGTTGGCTGTTAGTTGCCGGACGAAGCCGACGACCCCGGG247060.17925402724797618No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA239650.17387771241794497No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGTCCCCGGCCGC229570.16656418293255842No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG217180.15757463627343749No Hit
GCCCGGTATAGGTGCGACGCTCTAGCGCCATCCATTTTCAGGGCTAGTTG199790.14495734681402558No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC191070.13863056337031818No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG187740.1362144866653244No Hit
GTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGTC186160.13506811994043247No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTACCAAGCATGTT184300.13371859961872423No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT183590.13320346014108292No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC181530.13170883010736306No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA178870.12977887093760826No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA178500.12951041797038673No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAG178500.12951041797038673No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC177450.12874859198232563No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCGACTAGAGGCTGTTCACCTT175770.12752967040142787No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA170550.12374230691792414No Hit
CGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCC169040.12264672859223626No Hit
GCCGCTTGTCACCCTGGGTGCTCGTATTGCGGCGACGGCCCGGTATAGGT167600.12160193866575249No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGCTCGG163190.11840226951589587No Hit
CGACGCTCTAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAG163050.11830069271748772No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT161040.11684234011177076No Hit
GTCGGATTCCCCTGGTCCGTGCCAGTTCTAAGTTGGCTGTTAGTTGCCGG158650.11510827905323168No Hit
GCGAGCTTATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGGGATAAT158460.11497042482682063No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCTCAGT141960.1029988735858605No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGAC141770.10286101935944945No Hit
GCGAAATGAAAGTGAAGGCAGCCTCGGGTTCTGTCTAGGTGGGATCCTCG140660.10205566045778487No Hit
CTCGCTTCGTACTCCAGCCCGACCGGCCCAGCCCTTAGAGCCAATCCTTT139560.10125755704172086No Hit
GCGACCGTTCCCATTCTCGCTTCGTACTCCAGCCCGACCGGCCCAGCCCT139390.10113421378651097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTAT50400.042.290783145
CTTGTCT24750.039.8689421
TATTCCC46950.036.4363146
AGGCTTA29600.035.998834
GAAAGGC33850.033.22341
ATTCCCG51950.032.6506237
AAGGCTT34550.031.051623
GTTAATA57000.030.4224431
AAAGGCT37000.029.0017092
ATATTCC61650.028.6889615
TTGTCTC37650.028.1158662
TTACTTC48300.028.0650043
TCCTCTA53700.025.9176718
CGCAAGT62400.024.2842832
GGCTTAG44550.024.0808625
TATACGC80150.023.3333647
TACGCTA81150.022.8695059
TCCAGTC305900.022.7485087
TTGACTC308800.022.469752
GACTCCA309350.022.3777284
Produced by FastQC (version 0.11.5)