FastQCFastQC Report
Wed 2 Jan 2019
Hp-R-3_S18_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-R-3_S18_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13782675
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT443700.32192589609781846No Hit
CTCGGTTAAGGTGCCTAAGGCTGACGCTCATGAGATACCATAAAAGGTGT298260.21640211352295544No Hit
GGTTAAGGTGCCTAAGGCTGACGCTCATGAGATACCATAAAAGGTGTTGG293190.21272358232346045No Hit
GCCAGTTCTAAGTTGGCTGTTAGTTGCCGGACGAAGCCGACGACCCCGGG259690.1884177055615111No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGTCCCCGGCCGC235970.17120769371693087No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA208430.15122608637292834No Hit
GTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGTC203680.14777973071265194No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC201770.1463939329629408No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTACCAAGCATGTT199490.14473968224600814No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC199210.14453652864919184No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT197030.14295483278826498No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA194810.1413441149849358No Hit
GCCCGGTATAGGTGCGACGCTCTAGCGCCATCCATTTTCAGGGCTAGTTG194120.14084348647849565No Hit
CGACGCTCTAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAG192750.13984948495121594No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA189070.13717946625020178No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT188490.13675864808536806No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAG187300.1358952452988988No Hit
GCCGCTTGTCACCCTGGGTGCTCGTATTGCGGCGACGGCCCGGTATAGGT184800.13408137389875333No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGCTCGG179550.13027224395844783No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG177780.12898802300714485No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA174090.12631074882053012No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC172160.12491044009961781No Hit
CGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCC157280.11411427752595198No Hit
GCCAAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCA156040.11321459731147981No Hit
GCGAGCTTATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGGGATAAT154790.11230766161140708No Hit
GTCGGATTCCCCTGGTCCGTGCCAGTTCTAAGTTGGCTGTTAGTTGCCGG152000.11028338112884474No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCGACTAGAGGCTGTTCACCTT151990.11027612564324416No Hit
GCGAAATGAAAGTGAAGGCAGCCTCGGGTTCTGTCTAGGTGGGATCCTCG151270.10975373068000224No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGAC150850.1094490002847778No Hit
CGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTA147340.10690232483897358No Hit
GCGACCGTTCCCATTCTCGCTTCGTACTCCAGCCCGACCGGCCCAGCCCT142460.1033616478658896No Hit
GGCGAAATGAAAGTGAAGGCAGCCTCGGGTTCTGTCTAGGTGGGATCCTC140550.10197585011617845No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA140000.10157679840814646No Hit
GAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCC139520.10122853509931853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA45450.040.3645481
TATTCCC43100.039.8726466
GAAAGGC30000.036.0145841
AGGCTTA30850.034.5514374
ATTCCCG50450.034.2017947
CTTGTCT25000.033.0657421
ATATTCC60500.028.8845525
AAAGGCT37350.028.7326092
AAGGCTT37800.028.3905543
TATACGC79450.027.2841457
TACGCTA79300.027.244239
TTACTTC49050.026.7573783
TAATATT63500.026.6064013
CGCAAGT61900.026.356992
ATATACG85300.026.0971646
ATACGCT86150.025.1621288
TTGTCTC34900.024.7243842
CTTTAAT91750.024.6582321
TCCTCTA54400.024.2551528
CGGAAGC14350.022.735098145
Produced by FastQC (version 0.11.5)