FastQCFastQC Report
Wed 2 Jan 2019
Hp-R-2_S17_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-R-2_S17_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13579238
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGTCCCCGGCCGC317610.2338938311560634No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG269400.19839110265244633No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC267580.19705082126110465No Hit
GCCAGTTCTAAGTTGGCTGTTAGTTGCCGGACGAAGCCGACGACCCCGGG253630.18677778532197464No Hit
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT247240.18207207208534087No Hit
CTCGGTTAAGGTGCCTAAGGCTGACGCTCATGAGATACCATAAAAGGTGT231340.17036302036977333No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG225070.1657456773347665No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC220050.16204885723337348No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA203580.14992004706007803No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC198610.14626004787602956No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGGCA181910.1339618614829492No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT177980.1310677373796674No Hit
GTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGTC177180.13047860270215456No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA169890.12511011295331886No Hit
GCCAAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCA166200.12239272925329095No Hit
GTTCGATTAGTCTTTCGCCCCTATGCTCAAGTTTGACGATCGATTTGCAC156880.11552931026026644No Hit
GGTTAAGGTGCCTAAGGCTGACGCTCATGAGATACCATAAAAGGTGTTGG156720.11541148332476388No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA148470.10933603196291279No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAG146920.10819458352523167No Hit
GTCGGATTCCCCTGGTCCGTGCCAGTTCTAAGTTGGCTGTTAGTTGCCGG145410.10708259182142621No Hit
GTCACAATGTCTCCCTCGGATTTTCAAGGGACGACGGAGGTGCACCGGAC144750.10659655571247813No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT142890.10522681758726078No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA142700.1050868981013515No Hit
GCTGATTCCACCAGGCCCGTTCCCCTGGCTGTGGTTTCGCTAGATAGTAG139700.10287764306067837No Hit
GGGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC139210.10251679807070176No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGG137550.10129434361486261No Hit
ATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGGCAGCCTCGG137170.10101450464304403No Hit
GTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTC136530.10054319690103376No Hit
GCCCGGTATAGGTGCGACGCTCTAGCGCCATCCATTTTCAGGGCTAGTTG136280.100359092314311No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCC59750.038.9423876
GTTAATA66550.036.6383861
AGGCTTA37650.033.884794
ATTCCCG68700.033.6583337
CGCAAGT69450.032.677642
GAAAGGC43100.030.811931
ATATTCC80500.029.3545595
CCGTTCT152900.029.1639422
AAGGCTT44250.028.994813
AAAGGCT45400.027.788942
TATACGC75650.027.5957537
GTTCTTA163800.027.4811424
CTTGTCT40650.027.3134161
TACGCTA76550.027.0841189
TCTTAGT170650.026.6752686
CAAACTC204950.026.0309583
TAATATT91900.025.3980663
CTTTAAT91150.025.3171921
GGCTTAG50700.025.3057465
ATATACG85400.024.9542736
Produced by FastQC (version 0.11.5)