FastQCFastQC Report
Wed 2 Jan 2019
Hp-R-2_S17_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-R-2_S17_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13579238
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGTCCCCGGCCGC337600.24861483391041528No Hit
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT283800.2089955268476773No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC261560.19261758281282057No Hit
GCCAGTTCTAAGTTGGCTGTTAGTTGCCGGACGAAGCCGACGACCCCGGG254300.18727118561439163No Hit
CTCGGTTAAGGTGCCTAAGGCTGACGCTCATGAGATACCATAAAAGGTGT247910.1825654723777579No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC232370.1711215312670711No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC217170.1599279723943273No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG206060.15174636456036783No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT199440.14687127510394912No Hit
GCCAAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCA197180.1452069696399754No Hit
GTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGTC192990.1421213767665019No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGGCA190520.14030242344968105No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA185330.13648041222931653No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA172390.1269511588205465No Hit
GGTTAAGGTGCCTAAGGCTGACGCTCATGAGATACCATAAAAGGTGTTGG171960.12663449893138334No Hit
GTCACAATGTCTCCCTCGGATTTTCAAGGGACGACGGAGGTGCACCGGAC159040.11711997388955109No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA158860.11698741858711072No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT153250.11285611166105197No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA152390.11222279188272566No Hit
GTTCGATTAGTCTTTCGCCCCTATGCTCAAGTTTGACGATCGATTTGCAC151360.1114642809854279No Hit
ATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGGCAGCCTCGG149760.11028601163040223No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAG148390.10927711849516152No Hit
GCTGATTCCACCAGGCCCGTTCCCCTGGCTGTGGTTTCGCTAGATAGTAG147790.10883526748702689No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA146730.10805466403932239No Hit
GGGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC144940.10673647519838741No Hit
CGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCC142180.10470396056096816No Hit
CGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTA141850.10446094250649411No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGG139700.10287764306067837No Hit
GTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTC135920.10009398170943024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA59150.040.085431
TATTCCC57200.039.4230236
GAAAGGC39250.034.5458221
AGGCTTA37850.033.907284
ATTCCCG65950.033.858957
AAGGCTT41700.031.4719243
CGCAAGT64500.030.8012542
CAAGTCA109500.030.1283152
ATATTCC77200.029.9611825
CCGTTCT151750.029.5760042
CACCTAA14800.029.3917569
TATACGC79250.029.1829367
AAAGGCT44500.029.165582
AAGTCAT118700.028.5874823
CTTGTCT41800.028.4486141
TACGCTA81300.028.3578019
GTTCTTA161000.028.3276354
TAATATT81000.027.6601313
GAGTAGC14250.026.964787145
ATATACG87500.026.7657876
Produced by FastQC (version 0.11.5)