FastQCFastQC Report
Wed 2 Jan 2019
Hp-R-1_S16_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-R-1_S16_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221565
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68970.31045681760380633No Hit
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT66830.30082396868873973No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA46180.20787147798961544No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGTCCCCGGCCGC45180.2033701467208927No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC43640.19643809656705974No Hit
GCCAGTTCTAAGTTGGCTGTTAGTTGCCGGACGAAGCCGACGACCCCGGG37950.17082552164802742No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC37840.1703303752084679No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC37220.1675395498218598No Hit
GCGAGCTTATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGGGATAAT37100.1669993900696131No Hit
GGTTAAGGTGCCTAAGGCTGACGCTCATGAGATACCATAAAAGGTGTTGG36260.16321827180388598No Hit
GTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGTC34550.15552099533437014No Hit
CTCGGTTAAGGTGCCTAAGGCTGACGCTCATGAGATACCATAAAAGGTGT33640.15142478387983246No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA32800.14764366561410536No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA32370.1457080931685546No Hit
GGCGAAATGAAAGTGAAGGCAGCCTCGGGTTCTGTCTAGGTGGGATCCTC32110.1445377470386867No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG31680.14260217459313593No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAG31260.1407116154602724No Hit
GCGAAATGAAAGTGAAGGCAGCCTCGGGTTCTGTCTAGGTGGGATCCTCG30260.13621028419154965No Hit
GTCGGATTCCCCTGGTCCGTGCCAGTTCTAAGTTGGCTGTTAGTTGCCGG30220.13603023094080074No Hit
GCCCGGTATAGGTGCGACGCTCTAGCGCCATCCATTTTCAGGGCTAGTTG28660.12900815416159328No Hit
GAAACCTAAAGGCGAAATGAAAGTGAAGGCAGCCTCGGGTTCTGTCTAGG28300.12738767490485312No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCGACTAGAGGCTGTTCACCTT28280.12729764827947865No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGCTCGG28260.1272076216541042No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTACCAAGCATGTT26230.11806991917859706No Hit
GCCGCTTGTCACCCTGGGTGCTCGTATTGCGGCGACGGCCCGGTATAGGT25100.1129834148449404No Hit
GAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCC24860.11190309534044693No Hit
GTTCGATTAGTCTTTCGCCCCTATGCTCAAGTTTGACGATCGATTTGCAC24840.11181306871507248No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG24020.10812197707471985No Hit
GCAGAACTGGCGCTGTGGGATGAACCAAACGCTCGGTTAAGGTGCCTAAG23750.10690661763216472No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCTCAGT23620.10632144456723075No Hit
GCCAAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCA23390.10528613837542454No Hit
GCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAACA23380.1052411250627373No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG23200.10443088543436721No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGGCA22730.10231525973806753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAGT8950.040.501652
GTTAATA8450.031.7661191
GCAGGCT8150.031.155131
TATTCCC7600.030.5178956
CTTGTCT4600.029.9650421
AGTGCTA2451.9608706E-929.6109981
AGGCTTA5400.029.530224
GAAAGGC5900.029.5106241
ATTCCCG8250.028.1140887
AGGCTCC9050.027.2312813
CAGGCTC9100.027.0871472
GCGAGCT25450.025.9401661
AAAGGCT6350.025.1174012
CAAGTCA15400.024.9505942
TCCACTC10550.024.7330677
CCCTACT16450.024.67467
TATACGC12050.024.66187
AAGTCAT15950.024.539793
CACGAGC3251.4879333E-924.538872145
GAGCTTA26050.024.485713
Produced by FastQC (version 0.11.5)