FastQCFastQC Report
Wed 2 Jan 2019
Hp-R-1_S16_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-R-1_S16_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221565
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT93800.4222248730061916TruSeq Adapter, Index 27 (97% over 39bp)
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT78930.35529007704028465No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGTCCCCGGCCGC45790.2061159587948136No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC44680.20111948108653133No Hit
GCCAGTTCTAAGTTGGCTGTTAGTTGCCGGACGAAGCCGACGACCCCGGG40860.18392439564001054No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA40380.18176375663102362No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC37580.1691600290786No Hit
GTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGTC37390.16830477613754266No Hit
GGTTAAGGTGCCTAAGGCTGACGCTCATGAGATACCATAAAAGGTGTTGG37130.16713443000767478No Hit
CTCGGTTAAGGTGCCTAAGGCTGACGCTCATGAGATACCATAAAAGGTGT37050.16677432350617694No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC36740.1653789108128729No Hit
GCGAGCTTATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGGGATAAT35840.16132771267102244No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA33580.15115470400370912No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAG32500.14629326623348857No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA31620.14233209471701255No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGCTCGG30240.13612025756617518No Hit
GTCGGATTCCCCTGGTCCGTGCCAGTTCTAAGTTGGCTGTTAGTTGCCGG29810.13418468512062443No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTACCAAGCATGTT29610.1332844188668799No Hit
GGCGAAATGAAAGTGAAGGCAGCCTCGGGTTCTGTCTAGGTGGGATCCTC29330.1320240461116375No Hit
GCGAAATGAAAGTGAAGGCAGCCTCGGGTTCTGTCTAGGTGGGATCCTCG28470.12815290122053596No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG27750.12491194270705562No Hit
GAAACCTAAAGGCGAAATGAAAGTGAAGGCAGCCTCGGGTTCTGTCTAGG27620.12432676964212167No Hit
GAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCC26670.12005050493683507No Hit
CGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTA25470.11464890741436781No Hit
GTTCGATTAGTCTTTCGCCCCTATGCTCAAGTTTGACGATCGATTTGCAC25220.11352357459718712No Hit
GCCCGGTATAGGTGCGACGCTCTAGCGCCATCCATTTTCAGGGCTAGTTG24910.11212816190388306No Hit
GCCGCTTGTCACCCTGGGTGCTCGTATTGCGGCGACGGCCCGGTATAGGT24880.1119931219658214No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCTCAGT24100.10848208357621766No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA23960.10785189719859649No Hit
GCAGAACTGGCGCTGTGGGATGAACCAAACGCTCGGTTAAGGTGCCTAAG23900.10758181732247313No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT23750.10690661763216472No Hit
GCCAAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCA23350.10510608512467562No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGAC23000.10353061918062266No Hit
GTTAAGGTGCCTAAGGCTGACGCTCATGAGATACCATAAAAGGTGTTGGT22860.10290043280300148No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCGACTAGAGGCTGTTCACCTT22850.10285541949031425No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG22800.10263035292687812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA1550.060.8163644
TCTTCCG2000.050.7582743
TATACGC13300.040.3385627
TACGCTA13750.039.0183949
CGATCTG2109.094947E-1237.97648
ATACGCT14350.037.3869638
ATATACG14800.037.2357986
CGCAAGT8000.037.161472
CTTTAAT15850.034.7690731
TCCGATC2800.033.6662036
GCTAGAC900.003626282832.2274741
CTACGTT900.003629081932.2223978
AATATAC17400.032.0885625
CTCGATG11150.029.91115145
CGGCTCG3001.8189894E-1129.0047263
TATTCCC6950.028.170066
TTAATAT22950.027.4881423
AGCCGGT1855.812961E-627.4369036
TAATATA19650.027.3072474
GGCTCGG3204.1836756E-1127.1919334
Produced by FastQC (version 0.11.5)