FastQCFastQC Report
Wed 2 Jan 2019
Hp-A-neg_S27_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-A-neg_S27_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11725902
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT258150.22015363935328813No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG159120.13569958200230567No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA151490.1291926198939749No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG125700.10719857628010196No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGGT36800.053.970186
GCCGGTA38150.052.0608027
TACGCTA49050.048.6237839
TATACGC63950.037.064837
CCGGTAC54800.036.3754848
CTCTCTC120450.036.167837
TCTCTCT121150.035.8393448
AAGCCGG57550.035.7704285
GCCATCC57500.031.3893723
CTTGAAC156250.029.8632321
TTGAACT166900.028.2394562
ATATACG93000.027.9809596
ACCTGGC55500.026.2515036
GAACTCT174350.025.8594654
ATACGCT94250.025.6105888
CGGTACC81100.025.2069389
ACTCTCT180700.025.1914166
TGAACTC194600.024.7702243
CACTTCA83450.023.713054
CTTTAAG136800.023.4467141
Produced by FastQC (version 0.11.5)