FastQCFastQC Report
Wed 2 Jan 2019
Hp-A-neg_S27_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-A-neg_S27_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11725902
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT268840.2292702088078171No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA162430.1385223925630625No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG139280.11877977489492919No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT136020.11599960497708406TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGGT39500.054.8873256
TACGCTA51750.053.3762139
GCCGGTA42400.051.1256077
TATACGC71300.039.147537
CTCTCTC118650.036.9064567
TCTCTCT118800.036.798838
CCGGTAC59700.036.674638
AAGCCGG61600.036.608145
ATATACG88250.033.9338656
GCCATCC56550.030.9017033
ATACGCT97600.029.0442458
CTTGAAC165400.028.8032041
TTGAACT173700.027.8433912
CGGTACC82500.027.2421179
ACCTGGC56300.025.5008586
ACTCTCT185850.025.3989876
GAACTCT185350.025.154544
CACTTCA80350.024.7265014
AGGACTG17600.024.3073333
AACTCTC189500.023.9914385
Produced by FastQC (version 0.11.5)