FastQCFastQC Report
Wed 2 Jan 2019
Hp-A-3_S21_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-A-3_S21_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11604561
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT515540.44425635747875336No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA408320.35186165163852384No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT328340.28294047487018253No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA318110.27412497551609233No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG293800.25317631576067373No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA292040.2516596707105077No Hit
GCCAGTTCTAAGTTGGCTGTTAGTTGCCGGACGAAGCCGACGACCCCGGG270960.23349439931420068No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT269310.23207254457967No Hit
GCGGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTCTGCCCT264000.22749675752490767No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGG238880.20585009635435583No Hit
TCTTGCTCCGCCCCTTCGACGTAATGCGAACGGGACGGGCCGGTGGTGCG216300.18639222974483913No Hit
TGAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTT207860.17911922734517918No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCTCAGT206160.17765428610354153No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG182180.15698999729502908No Hit
CTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC181330.15625752667421025No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGGCA179720.15487014114536518No Hit
CTTGTCTCAAAGACTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG174630.15048393472187357No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCT162620.14013455571477457No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT155380.1338956294856824No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA154470.13311145505633518No Hit
GAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTTC151570.13061243764412975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150930.13006093035316027No Hit
GGACAAGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT148870.1282857662603523No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG147580.12717413437699196No Hit
GAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG146060.12586430456093944No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC137820.11876364818970747No Hit
CTCGGCTCGGGGGTCGATGAAGAGCGCAGCCAGCTGCGTGAATTAATGTG135180.1164886806144584No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGTCCCCGGCCGC135170.11648006331303701No Hit
CCCTTCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC134920.11626463077750206No Hit
CCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCAT130310.11229205482223756No Hit
CTTGCTCCGCCCCTTCGACGTAATGCGAACGGGACGGGCCGGTGGTGCGC127530.10989644502708892No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA126310.10884513425367837No Hit
GGCCTTTACAGGCACCTGCCTCGGTCGCTCTGGACTGTCCTAGGAGTCGG122150.10526033686237679No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGA120070.103467938166726No Hit
CTGAACTTAAGCATATCACTAAGGGCAGGAAAAGAAACTAACTAGGATTC117970.10165830486823242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGCT32550.069.110841
CGGCTCG32000.067.504363
GGCTCGG35950.060.6922344
TATACGC87300.055.549157
TACGCTA87850.055.040859
ATATACG94700.051.66756
GTTAATA92850.051.425611
ATACGCT94350.051.1690378
CTTTAAT99150.050.280491
TATTCCC95300.050.277336
GCTCGGG44950.049.023655
CTTGTCT54300.047.7093931
CTCTTAG32650.046.180128
ATTCCCG108750.043.9267
TTGAACT193200.042.8592222
ATATTCC114500.042.7959825
TTGTCTC61850.042.7896272
CGGGTCA9250.042.317263
TAATATA117000.042.067774
GCGCCAT150550.041.4527321
Produced by FastQC (version 0.11.5)