FastQCFastQC Report
Wed 2 Jan 2019
Hp-A-3_S21_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-A-3_S21_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11604561
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT562180.4844474513081538No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA371970.3205377609717421No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT352010.3033376273346316No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA351280.30270856433086957No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG320160.27589152230747893No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA287590.2478249715779856No Hit
GCCAGTTCTAAGTTGGCTGTTAGTTGCCGGACGAAGCCGACGACCCCGGG282140.24312854230332365No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT268900.23171923522139268No Hit
GCGGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTCTGCCCT268380.23127113554748No Hit
TGAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTT238840.20581562714867027No Hit
TCTTGCTCCGCCCCTTCGACGTAATGCGAACGGGACGGGCCGGTGGTGCG232220.20011097360770475No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGG232160.20005926979917635No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG223490.1925880694668243No Hit
CTCGGCTCGGGGGTCGATGAAGAGCGCAGCCAGCTGCGTGAATTAATGTG207790.1790589062352294No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA205330.17693905008556549No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCTCAGT203080.17500015726575094No Hit
CTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC195910.16882155214660857No Hit
CTTGTCTCAAAGACTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG186040.16031627564368872No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGGCA180490.1555336733548128No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCT173400.1494240066470416No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT172200.14838993047647386No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA166260.14327125343216343No Hit
GGACAAGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT156520.13487800184772178No Hit
GAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG155410.13392148138994658No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG155170.13371466615583305No Hit
CCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCAT142560.12284824906345015No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC140510.12108170227206354No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGTCCCCGGCCGC139610.12030614514413772No Hit
CCCTTCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC138790.11959952642758309No Hit
CTGAACTTAAGCATATCACTAAGGGCAGGAAAAGAAACTAACTAGGATTC137760.11871194438117909No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGA137580.11855683295559392No Hit
CTCGCTCTGACTGACGCCCGCAATGAGCAACGGGCGCGTGTAGTCACCTG131710.11349847702123328No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA128190.1104651869209012No Hit
GAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTTC126850.10931046853043386No Hit
CAGGCACCTGCCTCGGTCGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG118760.10233907168052285No Hit
GGCCTTTACAGGCACCTGCCTCGGTCGCTCTGGACTGTCCTAGGAGTCGG117680.10140840312701185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGCT39050.079.1042941
CGGCTCG38300.078.190743
GGCTCGG42700.070.133614
GTTAATA97100.059.4436341
TATACGC89050.058.9437267
TACGCTA90100.058.1763469
ATATACG94650.055.695216
GCTCGGG54700.055.2780575
TATTCCC104000.055.010776
GTCTATC7400.053.8942571
ATACGCT98550.053.3352478
CTTTAAT100300.052.9925161
ATTCCCG115550.049.5039677
TAATATT118350.048.4632153
TAATATA109850.048.1866654
ATATTCC124500.046.7681585
CTTGTCT52650.046.2756421
AATATAC116500.045.6228145
TTGAACT204550.044.9846462
AACTCTC217350.041.935295
Produced by FastQC (version 0.11.5)