FastQCFastQC Report
Wed 2 Jan 2019
Hp-A-2_S20_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-A-2_S20_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11397184
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT517060.45367346881475284No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT471760.4139268085871037No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA463220.4064337295949596No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA383780.3367323015930953No Hit
CTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC315630.2769368293080115No Hit
GCCAGTTCTAAGTTGGCTGTTAGTTGCCGGACGAAGCCGACGACCCCGGG312350.2740589254328087No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA284800.24988628770054078No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG269180.2361811479046052No Hit
CTTGTCTCAAAGACTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG206500.18118510677725302No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCTCAGT199530.17506956104244698No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC192440.16884872614147495No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCT192280.168708340586587No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT187590.16459328900893413No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG175580.15405559829515783No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG173950.1526254204547369No Hit
GCGGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTCTGCCCT167120.14663271208045775No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGTCCCCGGCCGC160270.14062245551181768No Hit
ATCAACGCGAGCTTATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGG152690.1339716898490013No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATC148150.12998824972905587No Hit
GAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG144090.12642596627377428No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT141500.1241534751040257No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA139190.122126658655331No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGGCA132570.11631820632184231No Hit
GGACAAGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT132550.1163006581274813No Hit
CTCGTGTTCCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCATTATT132230.11601988701770542No Hit
TCTTGCTCCGCCCCTTCGACGTAATGCGAACGGGACGGGCCGGTGGTGCG130990.11493189896732386No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGG129100.11327359460021001No Hit
AAAGAAACTAACTAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAG125200.10985169669981637No Hit
CTCGCTCTGACTGACGCCCGCAATGAGCAACGGGCGCGTGTAGTCACCTG120880.10606128671784187No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGA119080.1044819492253525No Hit
CTTGCTCCGCCCCTTCGACGTAATGCGAACGGGACGGGCCGGTGGTGCGC114860.10077928021518298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC100950.078.482977
TACGCTA103250.077.092149
ATATACG108200.073.559146
ATACGCT116250.068.403628
CTTTAAT121300.067.722861
GTTAATA115150.063.399181
TAATATA133200.060.3521424
TATTCCC122800.059.2057846
AATATAC144250.056.1305055
ATTCCCG138250.052.2747087
TTGAACT170150.051.818312
CTTGTCT62800.051.306571
TAATATT146300.049.7953763
TGAACTC178600.049.191323
TTAATAT228100.048.4949533
ATATTCC153050.048.0722355
AACTCTC182750.047.4389845
CGGCTCG23800.045.9904143
CTTGAAC199500.044.9235081
TTTAATA183400.044.397862
Produced by FastQC (version 0.11.5)