FastQCFastQC Report
Wed 2 Jan 2019
Hp-A-2_S20_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-A-2_S20_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11397184
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT533960.46850169304979195No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA506670.44455718184421694No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT503910.44213553102239994No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA367720.32264110152121783No Hit
GCCAGTTCTAAGTTGGCTGTTAGTTGCCGGACGAAGCCGACGACCCCGGG332390.2916422161825237No Hit
CTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC297350.2608977796620639No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG290420.2548173303159798No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA263470.2311711384145417No Hit
CTTGTCTCAAAGACTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG210710.18487900169024207No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG209910.18417707391580235No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCTCAGT200010.1754907177071108No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCT198930.1745431152116172No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC194310.17048948231422778No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT178420.15654744189441883No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG176190.1545908182231681No Hit
ATCAACGCGAGCTTATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGG168980.14826469415603014No Hit
GCGGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTCTGCCCT168320.14768560374211734No Hit
TCTTGCTCCGCCCCTTCGACGTAATGCGAACGGGACGGGCCGGTGGTGCG158990.1394993710727141No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGTCCCCGGCCGC156520.13733216906913145No Hit
GAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG153590.134761358595246No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATC151860.13324343978302008No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA149950.13156758722154524No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT149060.13078669257248104No Hit
AAAGAAACTAACTAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAG148860.13061121062887113No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGGCA141120.12382005941116683No Hit
GGACAAGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT138760.12174937247656965No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGG134940.11839766735361999No Hit
CTCGTGTTCCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCATTATT131950.11577421229665152No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGA131450.1153355074376267No Hit
CTCGCTCTGACTGACGCCCGCAATGAGCAACGGGCGCGTGTAGTCACCTG129890.11396674827746925No Hit
TGAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTT121130.10628063914735429No Hit
CTTGCTCCGCCCCTTCGACGTAATGCGAACGGGACGGGCCGGTGGTGCGC120240.1054997444982901No Hit
GCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGC116520.10223578034714541No Hit
CTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCG116250.101998879723272No Hit
CTAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAGCCCTGCACCGA116120.10188481645992553No Hit
GCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCG115120.10100740674187589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC96400.079.944787
TACGCTA98900.078.290459
ATATACG104700.074.1039666
ATACGCT112050.069.102428
GTTAATA109900.068.355591
CTTTAAT117300.067.257831
TAATATA126450.061.7591364
TATTCCC122200.061.47426
AATATAC141500.055.2928965
CTTGTCT66650.054.8330421
CGGCTCG27150.054.7505383
ATTCCCG137350.054.473457
TAATATT144300.052.5114823
TTGAACT167050.051.523432
CTCGGCT30650.051.101691
ATATTCC151900.050.552495
GGCTCGG29550.050.5491754
GCCGGTA10750.049.9064987
AACTCTC176150.048.285835
TTGTCTC75800.047.6387832
Produced by FastQC (version 0.11.5)