FastQCFastQC Report
Wed 2 Jan 2019
Hp-A-1_S19_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-A-1_S19_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392485
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA49330.35425875323612105No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44670.3207934017242555No Hit
GCCAGTTCTAAGTTGGCTGTTAGTTGCCGGACGAAGCCGACGACCCCGGG40700.29228322028603543No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT40640.2918523359318054No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCTCAGT31980.2296613608046047No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT31650.22729149685633956No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA31600.22693242656114787No Hit
GCGGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTCTGCCCT31030.2228390251959626No Hit
CTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC29560.21228235851732694No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT27120.19475972811197248No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA26740.19203079386851563No Hit
GTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTGCTGCCTT22800.1637360546074105No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG22690.16294609995798878No Hit
GCGAGCTTATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGGGATAAT22080.15856544235665015No Hit
TCTTGCTCCGCCCCTTCGACGTAATGCGAACGGGACGGGCCGGTGGTGCG21840.15684190493973005No Hit
GAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTG21820.15669827682165338No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCT19980.1434844899585992No Hit
CTTGCTCCGCCCCTTCGACGTAATGCGAACGGGACGGGCCGGTGGTGCGC19010.1365185262318804No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG18690.1342204763426536No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGG18680.13414866228361527No Hit
GCAGAATTCACCAAGCGTTGGCTTGTTCACCCACTAATAGGGAACGTGAG18570.13335870763419355No Hit
ATCAACGCGAGCTTATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGG18280.13127609992208175No Hit
GAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTTC18140.130270703095545No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGGCA17880.12840353756054823No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA17760.12754176885208815No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATC17560.1261054876713214No Hit
GGACAAGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT16550.11885226770844928No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG16070.11540519287460906No Hit
GGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAG16040.11518975069749406No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC15760.11317895704442058No Hit
GGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGGCCGTCACCT15420.1107372790371171No Hit
GTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATCGGACTCGGGGA15320.11001913844673372No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA14710.1056384808453951No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT14400.10341224501520661No Hit
TGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTT14260.1024068481886699No Hit
GCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCG13990.10046786859463476No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA7500.071.59651
TATTCCC7550.069.130556
TATACGC8750.056.3359077
ATTCCCG9300.056.1221127
TACGCTA8900.055.3884169
ATATACG9400.052.4403426
ATATTCC10050.051.2125855
TAATATT10050.050.4912833
CTTTAAT9950.050.3207741
ATACGCT10000.049.293928
TTGAACT17300.046.9407422
ACTACGC3300.046.1306766
CTTGTCT3600.044.3446771
GCGCCAT19600.043.6864971
GACTACG3400.042.6417965
CGCCATC20350.041.6868862
GCCATCC20250.041.525743
CATCCAT20550.041.272285
GCGTCGG2500.040.59937145
TGAACTC19500.040.520643
Produced by FastQC (version 0.11.5)