FastQCFastQC Report
Wed 2 Jan 2019
Hp-A-1_S19_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHp-A-1_S19_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392485
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA46750.3357307260042298No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT46430.33343267611500305No Hit
GCCAGTTCTAAGTTGGCTGTTAGTTGCCGGACGAAGCCGACGACCCCGGG44410.31892623618925875No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA37340.2681536964491539No Hit
GCGGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTCTGCCCT31480.22607065785268782No Hit
CTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC31400.22549614538038112No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT30700.22046916124769744No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCTCAGT30440.21860199571270064No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT29780.21386226781617035No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA28700.20610634944002987No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG27450.19712959206023764No Hit
GTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTGCTGCCTT27220.19547786870235587No Hit
TCTTGCTCCGCCCCTTCGACGTAATGCGAACGGGACGGGCCGGTGGTGCG23580.16933755121240085No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG23130.16610591855567564No Hit
GCGAGCTTATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGGGATAAT22290.16007353759645526No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA22000.15799092988434346No Hit
ATCAACGCGAGCTTATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGG21590.1550465534637716No Hit
GAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTG21240.15253306139742978No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATC20970.15059408180339465No Hit
CTTGCTCCGCCCCTTCGACGTAATGCGAACGGGACGGGCCGGTGGTGCGC20580.1477933335008995No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCTACGGTAATCCTGCT20120.14448988678513594No Hit
GCAGAATTCACCAAGCGTTGGCTTGTTCACCCACTAATAGGGAACGTGAG19040.13673396840899543No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGGCA18810.13508224505111366No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGAAATGAAAGTGAAGG18770.1347949888149603No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT18360.13185061239438844No Hit
GTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATCGGACTCGGGGA16810.12071943324344608No Hit
GAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTTC16370.1175596146457592No Hit
GGACAAGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT16120.11576426316980075No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC16000.11490249446134071No Hit
GAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG15880.11404072575288064No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA15620.11217356021788386No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGTCCCCGGCCGC15480.11116816339134712No Hit
GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA15130.10865467132500531No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT15020.10786471667558357No Hit
GCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCG14870.10678750579000851No Hit
CAGGCACCTGCCTCGGTCGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG14540.10441764184174337No Hit
GCCGAATCAACTAGCCCTGAAAATGGATGGCGCTAGAGCGTCGCACCTAT14420.10355587313328331No Hit
GGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAG13970.1003242404765581No Hit
GAACACTGTAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGGTGGGT13940.10010879829944308No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA6750.082.71121
TATTCCC6750.078.414516
TACGCTA9050.068.092769
CTTGTCT3450.067.252471
TATACGC9400.065.557397
ATTCCCG8450.062.632077
CTTTAAT10200.061.843841
ATACGCT10100.061.013818
ATATACG10300.060.5394636
TAAGTTA851.304943E-859.7112965
TAATATT9550.056.18313
GAAAGGC3600.052.3658561
AGGCTTA3700.050.9505654
AATATAC12150.050.1280025
ATATTCC10600.049.933775
TAATATA12350.049.903314
TTGTCTC4550.049.400082
AAGTTAG1056.904156E-848.3377156
AAAGGCT3750.048.3377152
TTGAACT16000.048.0356032
Produced by FastQC (version 0.11.5)