FastQCFastQC Report
Wed 2 Jan 2019
HCp-R-neg_S24_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCp-R-neg_S24_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9031860
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT224450.24850916643969237No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA212690.23548859260440264No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC197790.2189914369797583No Hit
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT195570.2165334715108516No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT190150.21053249275343064No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC150620.16676520672375347No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT149970.16604553214952403No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA145070.16062029305148662No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG134410.14881763003412363No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG134030.14839689720611257No Hit
CTCGGTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTG132540.14674718164364814No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC122730.13588563153104677No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG114620.12690630722796856No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG113640.12582125940836109No Hit
ATTCGCTTTACCGGATAACATTGAGCGAACATGAGTGCCAGCTATCCTGA113110.12523444783245088No Hit
ATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCTCGG108320.11993099981620618No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC103960.11510364421060555No Hit
GGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAG99140.10976698044478103No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG96650.10701007322965592No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA94090.10417566259884453No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG93650.10368849827167384No Hit
CTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG92210.10209414229184242No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACTA11500.062.412888145
TATACGC63800.054.990427
TACGCTA65100.054.1203969
CACTCGT8000.053.4591456
CGTGTCA12550.051.9919145
ATACGCT69550.050.44448
CTTGTCT38700.048.562231
CTAACTC14700.048.3249177
TATTCCC57550.045.4696736
ATATACG80400.045.169126
CTTTAAT90150.042.176971
GCCGGTA14200.041.348437
AGCCGGT14350.040.4108546
TTGTCTC46250.040.1325152
GTTAATA71650.039.293921
GTAGCTC18750.038.6997831
GTCGTTC15150.037.35871
AATATAC100950.037.1230965
TCCGCAC11200.036.8998832
GGAACGA19550.036.710829
Produced by FastQC (version 0.11.5)