FastQCFastQC Report
Wed 2 Jan 2019
HCp-R-neg_S24_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCp-R-neg_S24_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9031860
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT222880.24677087554501512No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA221290.2450104408172846No Hit
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT212670.23546644877134945No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC199460.22084044703970165No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT179600.19885162081786034No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA157200.17405052779826083No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT151180.16738523404924346No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG141660.15684476951591367No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG141350.15650154010358885No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC140710.15579293744588602No Hit
CTCGGTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTG128120.1418533945388879No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC124060.13735819642908548No Hit
ATTCGCTTTACCGGATAACATTGAGCGAACATGAGTGCCAGCTATCCTGA122340.1354538267865091No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG111920.12391688976578469No Hit
ATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCTCGG107600.11913382182629048No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC105430.11673121594001677No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG104720.11594510986662769No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG103950.11509257229407896No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT99540.1102098571058453No Hit
GGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAG97600.10806190529968356No Hit
CTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG95030.10521642275234558No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA92930.10289132028175813No Hit
ATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGG91220.10099802255570833No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAT8600.060.7061041
TATACGC62000.054.967
TACGCTA64550.052.788569
GCTAATC10900.051.2215732
ATACGCT66850.050.9723478
CTTGTCT39200.048.2783851
CTAACAT28950.048.089581
CCCGGTA8050.045.9380534
CTTTAAT81300.045.2184521
AATCTTA11800.044.857925
ATATACG81450.044.6010366
GTCGTTC15950.044.0909279
TATTCCC59800.042.681516
TTGTCTC46400.039.692132
AGGTATC12950.038.6293568
GACTGGC9950.038.623451
AATATAC100500.037.5176475
GTATAAC5950.036.5546388
GTATATG10350.036.4251378
GCCGGTA15550.035.9005367
Produced by FastQC (version 0.11.5)