FastQCFastQC Report
Wed 2 Jan 2019
HCp-R-3_S12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCp-R-3_S12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14274270
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT472010.33067190126009943No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA414600.29045268164326443No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT352800.24715799827241605No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC346450.24270943452800037No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC324880.22759832902137903No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA298160.20887933323385363No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG286680.20083689043292582No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTT275960.1933268741588887No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA256880.17996016608905394No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC254220.17809667324493653No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA240430.16843593402674883No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG236530.16570374527033604No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT236260.16551459374104596No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC235110.164708948338514No Hit
GGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAG232630.1629715565139233No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG229990.16112207489419775No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG228340.15996614888186927No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT223400.15650537645707976No Hit
CTCGCTTCGTACTCCAGCCCGACCGGCCCAGCCCTTAGAGCCAATCCTTT215700.15111105506621356No Hit
CTCGGTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTG209520.14678158672912872No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA199060.13945371637218576No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG197320.1382347398500939No Hit
GTCAGTCCCTCTTAATCATTACCTCGTGTTCCGAAAACCAACAAAATAGA197000.13801056025982414No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA195030.1366304546572259No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG190460.13342888988368581No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA183640.12865106236606144No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG181840.12739005217079402No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG178500.12505017769735335No Hit
GTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAGGACGGAAAC172950.1211620629286121No Hit
CTCGGTTCTATTTTGTTGGTTTTCGGAACACGAGGTAATGATTAAGAGGG171180.11992206956993247No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA169600.1188151828429755No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCT165320.11581678082311739No Hit
GGACAAGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT164460.1152142981742674No Hit
GTTTATGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACT163550.11457678746443775No Hit
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT163330.11442266399612729No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT162450.11380617012288544No Hit
ATTCGCTTTACCGGATAACATTGAGCGAACATGAGTGCCAGCTATCCTGA159990.11208278952268662No Hit
CTCCGCTTAACTGAATAAGTAAAGAAACGATGGGAGTAGTGGTATTTCAA157330.1102192966785692No Hit
CTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG156190.10942065688823316No Hit
GGGAGCTTCGCGGCGCAATTGAAATACCACTACTCCCATCGTTTCTTTAC156050.10932257831749015No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT154870.10849591607837038No Hit
CTTTGAGCAGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTC153660.10764823700266284No Hit
CCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTTT153510.10754315281972388No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA153280.10738202373921747No Hit
GGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCCAGAC150600.10550451967070819No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGAC150550.10546949160972856No Hit
GTTATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGC149580.10478994722672333No Hit
TTTTACTCTACGCGAGGTTTCCGTCCTCGCTGAGCTCACCTTAGGACACC149560.10477593600233147No Hit
CTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTAATTTGCCGACTTCC148970.10436260488277159No Hit
GTGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGAC148120.10376712784611754No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC146860.10288442070943032No Hit
CCAGAACTTGCCAAGGGGCTTGCGCCCCGCTCTCCGCTTAACTGAATAAG145380.10184759010443266No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTC144700.10137120847510941No Hit
CCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGCCCGAAGCTTC144020.10089482684578616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACAT72150.065.573491
TAACATG77700.060.6526952
TGTGCGC84150.055.8177227
ACATGTG87150.054.5618674
AACATGT90050.052.966473
ATGTGCG98200.049.1600656
AGTAGTC31150.047.9360164
TATACGC134350.046.7770357
GCTCGGG31200.046.4649125
TACGCTA136100.046.1812259
ATACGCT138300.045.5993848
ATATACG140650.045.1976
TATTCCC116750.044.8880926
CATGTGC108900.044.5960775
GTTAATA119500.044.14521
AATATAC149650.042.96325
AGATTAT41750.042.71781145
CTTTAAT153100.042.4668271
CCAGTAG34950.042.3195232
CGCCCTT15950.041.854691
Produced by FastQC (version 0.11.5)