FastQCFastQC Report
Wed 2 Jan 2019
HCp-R-3_S12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCp-R-3_S12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14274270
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT497250.34835406644262723No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA451040.3159811324852339No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT380130.26630433640389317No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC369430.2588083313542479No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC343580.24069882382776842No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTT325220.22783651983604067No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA312190.21870820714474365No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG298250.20894238374361704No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA274970.1926333185514916No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT272310.1907698257073742No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA265730.18616013288245212No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG249100.17450979980062029No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC243780.17078281411238544No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC240230.16829582178283023No Hit
GGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAG236880.16594894169719362No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT218840.15331081729573562No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG216230.15148235251259784No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG215070.15066970149786996No Hit
CTCGCTTCGTACTCCAGCCCGACCGGCCCAGCCCTTAGAGCCAATCCTTT209540.14679559795352057No Hit
CTCGGTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTG208030.1457377505119351No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG205850.14421052705322232No Hit
GTCAGTCCCTCTTAATCATTACCTCGTGTTCCGAAAACCAACAAAATAGA204130.14300556175552234No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG202710.14201076482370026No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA201350.14105800156505374No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA199780.13995812045029274No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA199470.1397409464722189No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG188600.13212584601524283No Hit
TTTTACTCTACGCGAGGTTTCCGTCCTCGCTGAGCTCACCTTAGGACACC188120.13178957662983817No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG185590.13001715674426784No Hit
GTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAGGACGGAAAC180650.12655638431947833No Hit
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT177920.12464385218998941No Hit
GTTTATGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACT175610.12302555577272953No Hit
CCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTTT174260.12207979812627896No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA170480.11943167671621736No Hit
ATTCGCTTTACCGGATAACATTGAGCGAACATGAGTGCCAGCTATCCTGA167400.11727394815987087No Hit
CTCGGTTCTATTTTGTTGGTTTTCGGAACACGAGGTAATGATTAAGAGGG167320.11721790326230343No Hit
CCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGCCCGAAGCTTC166470.11662242622564936No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT165990.11628615684024471No Hit
GGACAAGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT163900.11482198389129532No Hit
CTTCAGTGTAGCGCGCGTGCGGCCCCGAACATCTAAGGGCATCACAGACC160740.1126082104373814No Hit
GTTATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGC159360.11164143595434302No Hit
CCAGAACTTGCCAAGGGGCTTGCGCCCCGCTCTCCGCTTAACTGAATAAG158540.11106697575427675No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT158250.11086381300059478No Hit
CTTTGAGCAGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTC158110.11076573442985176No Hit
CTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG154970.1085659722003297No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC153830.10776733240999364No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGAC152020.10649931660253029No Hit
CTCCGCTTAACTGAATAAGTAAAGAAACGATGGGAGTAGTGGTATTTCAA150800.10564463191462682No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCT149800.1049440706950338No Hit
CTCGTCGCCGCTTGGCACCCTGGGTGCTCGTATTGCGGCGACGGCTCGGT148390.10395627937540763No Hit
GGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCCAGAC147520.10334679111436171No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC145880.10219787071422917No Hit
CTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTAATTTGCCGACTTCC144510.10123810184338675No Hit
GAAACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGCTTCGCCT143780.10072669215308383No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTC143580.10058657990916524No Hit
GTGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGAC142970.1001592375652135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACAT71400.068.247581
TAACATG75850.064.910762
TGTGCGC81400.060.297367
ACATGTG87150.056.6609344
ATGTGCG91750.054.7685436
AACATGT90550.054.693573
GTTAATA110400.053.0711481
GCTCGGG39500.050.8495035
CATGTGC99250.050.8490375
TATACGC144200.049.975277
TACGCTA146200.049.2418569
TATTCCC118150.048.9748576
ATACGCT147700.048.692688
ATATACG149200.048.551286
AGTAGTC26950.047.085084
CGCCCTT14100.046.2849271
AATATAC158500.046.2057575
CTTTAAT161300.046.0343251
ATTCCCG127200.045.540077
TAATATT127550.045.251883
Produced by FastQC (version 0.11.5)