FastQCFastQC Report
Wed 2 Jan 2019
HCp-R-2_S11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCp-R-2_S11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13570418
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC393500.2899689604255374No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC354340.2611120748086021No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT330470.24352234396906566No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA285150.21012617297418548No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC279080.20565320832416512No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT258190.1902594304759072No Hit
GGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAG248780.18332522992291025No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG247430.18233041900404248No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC242740.17887437218219807No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG238390.17566887033251297No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG234090.17250021333167484No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG224570.16548495411121456No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA224460.16540389544375125No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA218480.16099725152165542No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA214570.15811598434182353No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA212120.1563105867483227No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG195210.14384965886828247No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA194660.14344436553096596No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT183150.13496268132639688No Hit
GTGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGAC182660.1346016018076967No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG180610.13309096300497153No Hit
CTCGGTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTG170630.1257367311751193No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG169250.12471981334694333No Hit
GCTGATTCCACCAGGCCCGTTCCCCTGGCTGTGGTTTCGCTAGATAGTAG168090.12386501285369397No Hit
GGGAGCTTCGCGGCGCAATTGAAATACCACTACTCCCATCGTTTCTTTAC166620.12278177429759349No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA163680.12061529718539253No Hit
GGACAAGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT163050.12015105208992088No Hit
TTTTACTCTACGCGAGGTTTCCGTCCTCGCTGAGCTCACCTTAGGACACC162260.119568903478139No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTT162170.11950258275021447No Hit
GTCAGTCCCTCTTAATCATTACCTCGTGTTCCGAAAACCAACAAAATAGA161850.11926677571759398No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG161590.1190751825035898No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCT157360.11595810829113737No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG156860.11558965980266783No Hit
GCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACC156170.11508120088857986No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA156060.11500014222111654No Hit
GTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAGGACGGAAAC155850.11484539385595934No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC154580.11390953469524667No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT153180.11287787892753194No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC149930.11048296375247985No Hit
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT146550.10799225197042567No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT144690.10662162359331895No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT144380.10639318553046781No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT140930.1038508909600279No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140210.10332032513663177No Hit
ATTCGCTTTACCGGATAACATTGAGCGAACATGAGTGCCAGCTATCCTGA138280.10189811397113928No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA138250.10187600706183111No Hit
GGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGC137860.10158861724082487No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGAC136850.10084435129411637No Hit
ATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCTCGG135710.10000428874040579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA103000.049.592051
TATTCCC108450.046.5855036
ATTCCCG116750.042.963377
AGGCTTA45000.042.685714
GTCATAT48750.042.268841
ATCGGGT33500.042.234411
ATATTCC121050.041.8562135
TAATATT123150.041.142773
TCATATG46700.041.1424562
CATATGC47150.040.8931663
TATACGC120200.038.4738247
GCTCGGG40550.038.4321945
GAAAGGC51600.038.3874781
AAAGGCT49650.038.2598462
TACGCTA120600.038.2305159
CTAACAT53450.037.6018031
ATACGCT122800.037.6011738
AAGGCTT50700.037.457963
ATATACG124450.037.451026
GGCTTAG52200.036.9367525
Produced by FastQC (version 0.11.5)