FastQCFastQC Report
Wed 2 Jan 2019
HCp-R-2_S11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCp-R-2_S11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13570418
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC405480.29879698620926787No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT357240.2632490760417255No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC332540.2450477207113296No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA328750.24225488116873037No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC289540.21336115070294814No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT271020.19971381869003593No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC260620.19205009012986926No Hit
GGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAG256690.18915408501049857No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG254220.187333949477459No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA248700.18326627816475513No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA231090.1702895224008575No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG226910.16720929303725207No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG222490.16395220839918123No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG221620.1633111080292442No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA218340.16089408594488394No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG217260.16009823720978972No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA211840.15610425559477978No Hit
TTTTACTCTACGCGAGGTTTCCGTCCTCGCTGAGCTCACCTTAGGACACC200880.1480278647275272No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG195150.14380544504966614No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTT194840.143577006986815No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT191380.1410273434466057No Hit
GCTGATTCCACCAGGCCCGTTCCCCTGGCTGTGGTTTCGCTAGATAGTAG184290.13580274388010746No Hit
GTGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGAC182180.13424789125876593No Hit
CTCGGTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTG179490.13226563839079974No Hit
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT177520.13081395134622972No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA177260.13062235813222556No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG175110.12903802963180647No Hit
GGACAAGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT172960.1274537011313874No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT171350.12626729699851547No Hit
ATTCGCTTTACCGGATAACATTGAGCGAACATGAGTGCCAGCTATCCTGA169340.12478613407486784No Hit
GTCAGTCCCTCTTAATCATTACCTCGTGTTCCGAAAACCAACAAAATAGA166670.12281861914644045No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA165330.12183117719734204No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC162190.11951732068975326No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG159780.11774139897533001No Hit
GTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAGGACGGAAAC159160.11728452284962777No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA156010.11496329737226961No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC154100.1135558241463159No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA152740.11255364425767872No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG152510.11238415795298273No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT151020.11128618145734347No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT150870.1111756469108026No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGAC150330.11077772254325549No Hit
ATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCTCGG145820.10745431717726012No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT144940.1068058478375537No Hit
GACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCGT142200.10478675012074057No Hit
GAAAGGCTTAGTCTCAATAGATCGCAGTGTGGTGGCTGCTCTACTAGGTA141690.10441093266250162No Hit
GCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACC141240.10407932902287903No Hit
GTTTATGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACT140800.10375509435302582No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCT139900.10309188707378063No Hit
GGGAGCTTCGCGGCGCAATTGAAATACCACTACTCCCATCGTTTCTTTAC138280.10189811397113928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA101900.053.0151251
TATTCCC111050.048.450326
AGGCTTA44850.047.856464
CTAACAT52550.047.1922951
ATTCCCG119250.045.110757
TAATATT121150.044.2914243
ATATTCC123350.043.560255
TATACGC125000.043.5576487
AAAGGCT49500.043.5073432
TAACATG58350.042.8735582
AAGGCTT50150.042.798853
GCTCGGG44900.042.7967575
TACGCTA126700.042.6864789
ATACGCT128750.042.006968
ATATACG130200.041.93686
ATCGGGT35650.041.4942931
GGCTTAG52000.041.27625
TGTGCGC61650.040.9242447
CATATGC44900.040.6972923
GAAAGGC53650.040.547981
Produced by FastQC (version 0.11.5)