FastQCFastQC Report
Wed 2 Jan 2019
HCp-R-1_S10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCp-R-1_S10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800706
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT47930.2661733786637019No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC46690.2592871906907624No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC40420.22446751440823765No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT39430.21896967078468113No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC38880.2159153132160386No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA35770.1986443095097145No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA34500.19159152021484907No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG34050.18909250038595973No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG33050.18353912298842787No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA32930.18287271770072405No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT32440.18015156277593344No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG32030.17787467804294538No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA30290.16821180137123995No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28410.15777145186388006No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA27970.15532796580896602No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG27780.15427282410343499No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG27660.15360641881573114No Hit
CTCGGTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTG26540.1473866361304955No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT26200.14549848781533467No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC25670.1425551977946428No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT25230.14011171173972875No Hit
GCGAACATGAGTGCCAGCTATCCTGAGGGAAACTTCAGAGGGAACCAGCT25110.13944530645202494No Hit
GGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAG24420.13561347604772794No Hit
GTTAATTCCGATAACGAACGAGACTCTAACCTACTAAATAGTTCACCGAT24080.13372532773256712No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC23700.131615044321505No Hit
GTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGTC23600.1310597065817518No Hit
GCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACC23590.13100417280777651No Hit
GGTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTGTTG23400.12994903110224545No Hit
GGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGC23190.12878282184876377No Hit
GCGGATTGCTAGTTGGCATCGTTTATGGTCAGAACTACGACGGTATCTGA22800.12661700466372633No Hit
GAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCTCGGGTTCTGCCTAGG22690.12600613314999784No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTC22520.12506205899241743No Hit
GCTCGGTATAGGTGCGACGCTCTAGCGCCATCCATTTTCAGGGCTAGTTG22460.12472885634856551No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA22390.12434011993073828No Hit
GCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGGGAGAAT22170.12311837690328127No Hit
GAACACTGTAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGGTGGGT22170.12311837690328127No Hit
GTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAGGACGGAAAC21030.11678752667009495No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAG21020.11673199289611964No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAG20830.11567685119058858No Hit
GAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAG20740.1151770472248107No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT20220.11228929097809415No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGCTCGG20190.1121226896561682No Hit
GCGAGGTTTCCGTCCTCGCTGAGCTCACCTTAGGACACCTGCGTTACCGT19810.11001240624510608No Hit
GGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAG19590.10879066321764909No Hit
CGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAGGACGGAAACCTCGCG19430.10790212283404398No Hit
GGACAAGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT19380.1076244539641674No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGAC19120.1061805758408091No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA19060.10584737319695718No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC19010.1055697043270806No Hit
GTGAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCA18890.10490329903937677No Hit
GTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCT18670.10368155601191978No Hit
GAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAG18650.10357048846396913No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA18620.10340388714204318No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCGACTAGAGGCTGTTCACCTT18240.10129360373098108No Hit
GTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTGCTGCCTT18240.10129360373098108No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT18190.10101593486110448No Hit
GAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTG18100.1005161308953266No Hit
GCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGA18030.10012739447749937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA7200.084.6761861
TATTCCC7550.081.6100166
ATATTCC8950.069.654135
ATTCCCG9050.067.282517
TAATATT9450.062.9004143
CTTGTCT4750.058.0636671
AGGCTTA2850.053.412874
TTGTCTC5050.053.12692
AAAGGCT3500.045.578372
CGACCGT700.001057663541.4222268
AAGGCTT3850.041.4222223
AAGTCAT12900.039.8969885
CAAGTCA13150.039.138494
GGCTTAG3900.039.0324825
GCTATAT4750.038.15947145
AATATTC17350.035.931094
TATACGC13850.035.0668267
CCGAATG1458.693205E-735.005332
CCGTATA3650.033.768513145
CTTTAAT15050.033.757951
Produced by FastQC (version 0.11.5)