FastQCFastQC Report
Wed 2 Jan 2019
HCp-R-1_S10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCp-R-1_S10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800706
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT57380.3186527950703779No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC47090.2615085416497751No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT41780.23202010766888098No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC38960.21635958340784112No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC38750.21519337415435946No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA38330.21286095564739607No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA36220.20114332933860385No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG35540.1973670327082822No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT34940.19403500626976308No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA32260.1791519548443777No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG31000.17215469932348756No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA30790.17098849007000586No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA30670.17032208478230204No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG30240.16793413250136335No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT29360.16304716039153533No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG29090.16154774849420173No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT27450.15244020956224946No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC27230.15121846653479248No Hit
CTCGGTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTG26890.14933031821963164No Hit
GCGAACATGAGTGCCAGCTATCCTGAGGGAAACTTCAGAGGGAACCAGCT25820.14338820440427255No Hit
GAACACTGTAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGGTGGGT25810.14333267063029723No Hit
GCGGATTGCTAGTTGGCATCGTTTATGGTCAGAACTACGACGGTATCTGA25600.14216646137681554No Hit
GGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGC24960.13861229984239515No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG24560.1363909488833824No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT24550.13633541510940708No Hit
GGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAG24430.13566900982170327No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA24420.13561347604772794No Hit
GAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCTCGGGTTCTGCCTAGG24360.13528027340387602No Hit
GTTAATTCCGATAACGAACGAGACTCTAACCTACTAAATAGTTCACCGAT24110.13389192905449307No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC24070.1336697939585918No Hit
GGTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTGTTG23950.13300338867088798No Hit
GTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGTC23920.13283678734896204No Hit
GCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGGGAGAAT22630.12567293050614592No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTC22330.12400691728688637No Hit
GCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACC22160.12306284312930596No Hit
GTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAGGACGGAAAC21970.1220077014237749No Hit
GTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCT21780.12095255971824384No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGAC21670.12034168820451534No Hit
GGACAAGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT21490.1193420802729596No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGCTCGG21240.11795373592357664No Hit
CGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAGGACGGAAACCTCGCG21240.11795373592357664No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAG21210.11778713460165069No Hit
GTGAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCA20770.11534364854673668No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAG20500.11384423664940306No Hit
GCTCGGTATAGGTGCGACGCTCTAGCGCCATCCATTTTCAGGGCTAGTTG20440.11351103400555115No Hit
GCGAGGTTTCCGTCCTCGCTGAGCTCACCTTAGGACACCTGCGTTACCGT20430.11345550023157584No Hit
GAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAG20310.11278909494387203No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC20310.11278909494387203No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGAA19960.11084541285473586No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA19860.11029007511498268No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA19790.10990133869715545No Hit
ATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCTCGG19780.10984580492318012No Hit
GAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAG19730.10956813605330354No Hit
GTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTC19240.10684698112851292No Hit
GAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCC19180.10651377848466101No Hit
GCTGATTCCACCAGGCCCGTTCCCCTGGCTGTGGTTTCGCTAGATAGTAG19160.10640271093671039No Hit
GTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATCGGACTCGGGGA19110.10612504206683379No Hit
GTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTGCTGCCTT19030.10568077187503123No Hit
GGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAG18530.1029040831762653No Hit
GGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAGGACGGAAACCTCGCGT18350.10190447524470957No Hit
TTTTACTCTACGCGAGGTTTCCGTCCTCGCTGAGCTCACCTTAGGACACC18180.10096040108712916No Hit
GAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTG18140.1007382659912279No Hit
ATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTC18020.10007186070352407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA7750.086.077151
TATTCCC8200.080.466886
TTGTCTC4450.068.4351042
TAATATT9650.067.624623
ATTCCCG9800.066.5803157
CTTGTCT4550.065.339261
ATATTCC10100.064.611645
AGGCTTA3250.058.0068974
ATGCCGG953.141031E-853.421474145
CTTCCGA701.6953394E-551.7918744
AAGGCTT4100.044.2125743
CGCCCTT1002.7326096E-643.5063821
GGCTTAG4550.041.43355
GAAAGGC4600.039.4079551
AAAGGCT4600.039.406862
AAGTCAT14550.038.87063
CAAGTCA14650.038.6052742
TGCACCC2109.094947E-1237.97649145
GTCATAT2750.036.9145051
AGCCGGT800.002035858336.2543146
Produced by FastQC (version 0.11.5)