FastQCFastQC Report
Wed 2 Jan 2019
HCp-A-neg_S25_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCp-A-neg_S25_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9885528
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA865970.8759977211131262No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT769650.7785623590363611No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT480450.4860134936646783No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG373680.3780071231400083No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG307860.3114249436145444No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATC289150.2924982863838937No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG281670.28493166981065654No Hit
CTAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAGCCCTGCACCGA281500.28475970125217387No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG265330.2684024566012053No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA250720.25362327636925414No Hit
CTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC228360.23100435303000508No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC225740.22835401406986053No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC212610.21507197187646426No Hit
AAAGAAACTAACTAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAG211370.2138176129792966No Hit
CCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCAT191190.19340393350764876No Hit
CTTTGAGCAGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTC176230.17827070036117443No Hit
CTCGTGTTCCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCATTATT167800.16974308301994592No Hit
GGCAAGTTCATCTACATCAGCCATAAGTTGTTTACATGCGTCATTAGTCA159440.16128627626162204No Hit
ATCGGACTCGGGGAAGTATTTAGCCTTAGATGGAGTTTACCACCCACTTT156540.158352694969859No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG155400.15719949404826933No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA147310.14901581382400617No Hit
CTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCG145510.1471949702636015No Hit
CTTGTTTGCTATCGGACTCGGGGAAGTATTTAGCCTTAGATGGAGTTTAC142410.14405907302068235No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT141280.14291598789665055No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT140770.14240008222120257No Hit
CTGAACTTAAGCATATCACTAAGGGCAGGAAAAGAAACTAACTAGGATTC138920.14052865967300887No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATCGGAC137940.1395373115123441No Hit
CTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG135560.13712975169358682No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG134650.1362092141158267No Hit
GTCACAATGTCTCCCTCGGATTTTCAAGGGACGACGGAGGTGCACCGGAC132580.13411524402136132No Hit
ATCCATTCCATCACCAAACCATTTAGTCTTACCACTCTTTCCTTCCTTTT132530.1340646650335723No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC132310.13384211748730063No Hit
CTTATGGAAAACTGATTGAGAGTCCTAACAAGCCCGGATATTTGCGCCCA126170.12763101778680916No Hit
CTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTGAC124560.12600237438000278No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT120950.12235057146163562No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA120230.12162223403747376No Hit
CTCGTTAAAGGATTTAAAGTGTACTCATTCCAATTACGGGGCCTCGAAGG115340.11667560903170777No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA112620.11392411209598516No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT112460.1137622593350603No Hit
CTCGGTTCTATTTTGTTGGTTTTCGGAACACGAGGTAATGATTAAGAGGG107510.10875493954394747No Hit
ATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTTCCGTC104050.1052548735889474No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTT100870.10203804996556581No Hit
CACCACGTGGGTATAATTTTGAAGCAGCTAAAACAGTTCCGGGATCACGT100050.10120855456582592No Hit
CTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTG99140.10028801698806578No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGCTTCGCCTGTAC98980.10012616422714092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA123800.0108.858959
TATACGC126350.0106.7680747
ATATACG130450.0104.1331946
ATACGCT139450.096.687718
CTTTAAT144700.095.583731
ACGTGGG16300.089.386545
GCCGGTA27350.087.198467
AGCCGGT28000.085.172926
AATATAC170000.080.930275
CTTGTCT77000.079.444981
TTTAATA183200.078.724372
TAATATA180150.075.768854
CGTGGGT19650.074.147616
GCTCTTA12850.070.51437
CCGGTAC35600.068.0100868
TTGAACT187000.067.120532
AAGCCGG35900.066.833965
CTTGAAC189250.066.7179951
TGTGTCG22300.065.9928059
GTTAATA121950.063.3721
Produced by FastQC (version 0.11.5)