FastQCFastQC Report
Wed 2 Jan 2019
HCp-A-neg_S25_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCp-A-neg_S25_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9885528
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA891430.9017525416952945No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT760410.7692153620929504No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT492570.4982738403047364No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG366620.3708653700641989No Hit
CTAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAGCCCTGCACCGA317890.32157108856502153No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG316660.3203268454654116No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG293020.29641310003876375No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATC287110.29043466368210175No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG274890.2780731590664656No Hit
AAAGAAACTAACTAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAG244290.24711881853958637No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA241630.24442801638921058No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC232720.23541484076520747No Hit
CTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC224770.22737278170675354No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC223220.22580483308529395No Hit
CCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCAT193880.19612508305069795No Hit
CTTTGAGCAGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTC178790.18086034453597216No Hit
CTCGTGTTCCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCATTATT170980.1729599066433275No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG166120.1680436290302349No Hit
AAACTACCATTGACACGCGAAGAAACCATTATCGGAATTAACGGAGACCC165380.16729506001095742No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA159440.16128627626162204No Hit
ATCGGACTCGGGGAAGTATTTAGCCTTAGATGGAGTTTACCACCCACTTT159410.16125592886894863No Hit
CTGAACTTAAGCATATCACTAAGGGCAGGAAAAGAAACTAACTAGGATTC152030.1537904702712895No Hit
CTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCG150700.15244506919610162No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT141610.14324980921605807No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATCGGAC140790.14242031381631817No Hit
CTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG140130.14175267117750312No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG138760.14036680691208403No Hit
CTTATGGAAAACTGATTGAGAGTCCTAACAAGCCCGGATATTTGCGCCCA138740.1403465753169684No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT136380.13795924709332671No Hit
GTCACAATGTCTCCCTCGGATTTTCAAGGGACGACGGAGGTGCACCGGAC135220.1367858145766215No Hit
CTTGTTTGCTATCGGACTCGGGGAAGTATTTAGCCTTAGATGGAGTTTAC134210.13576411902328334No Hit
ATCCATTCCATCACCAAACCATTTAGTCTTACCACTCTTTCCTTCCTTTT129400.13089842039797975No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT127660.1291382716229219No Hit
CTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTGAC126310.12777263895261842No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC125220.1266700170188178No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA122290.12370608833438133No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT121790.12320029845649114No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA114500.11582588203685226No Hit
CTAACAGCCAACTTAGAACTGGCACGGACCAGGGGAATCCGACTGTCTAA110330.11160759445524812No Hit
CTCGTTAAAGGATTTAAAGTGTACTCATTCCAATTACGGGGCCTCGAAGG109050.11031277236784925No Hit
CTCGGTTCTATTTTGTTGGTTTTCGGAACACGAGGTAATGATTAAGAGGG106020.10724768570783473No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGCTTCGCCTGTAC105590.10681270641284916No Hit
ATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTTCCGTC105300.10651934828367286No Hit
GTAAAACAATTGCCAGGAAAAAACACTATTGATGAATTTGTGACACGTTT103870.10507278923290693No Hit
CTAATACATGCAACGAAGCTCCGACCTCACGGGAAGAGCGCTTTTATTAG101070.10224036591672189No Hit
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT98960.10010593263202533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC124750.0110.944387
TACGCTA126750.0109.135499
ATATACG134000.0103.896836
ATACGCT139950.098.893718
CTTTAAT144350.097.858331
GCCGGTA26800.090.0843667
AAACTAC32150.080.97281
AATATAC174050.080.781085
AGCCGGT30000.080.729286
TGTGTCG18850.079.999569
CTTGTCT76500.077.538521
TTTAATA185950.076.858512
TAATATA190050.073.331644
AAGCCGG35850.068.1626745
CCGGTAC35600.067.8156368
ACGCGGA18400.067.377348
TTGAACT183750.066.650562
CTTGAAC184650.065.7795941
GTTAATA120100.064.001261
AACTCTC195350.062.3593755
Produced by FastQC (version 0.11.5)