FastQCFastQC Report
Wed 2 Jan 2019
HCp-A-3_S15_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCp-A-3_S15_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12880985
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT695100.5396326445531922No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA545300.42333719044001683No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT457770.35538431261273884No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG448210.3479625199470382No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA417490.32411341213424283No Hit
CCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCAT397110.3082916407402074No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG389590.30245357789020016No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT353550.2744743511462827No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG342340.26577160054141824No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT313340.243257794337933No Hit
CTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG305290.2370082722711035No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG295330.22927594434742374No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTC279210.21676137345086577No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA266770.20710372692771553No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA266090.20657581698915106No Hit
GCAGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTCTGCCCT262450.20374994614154118No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC257580.19996917937564557No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC235910.18314593177462749No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG232710.18066164971079465No Hit
TCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCG227520.17663245473851572No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT221020.17158625679635525No Hit
GAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTTC218410.1695600142380416No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC213610.1658335911422923No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG210330.16328720202686361No Hit
CTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC205580.15959959583836175No Hit
GAACACTGTAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGGTGGGT201850.15670385455770658No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG201540.15646318973277276No Hit
CTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCGC199940.15522104870085632No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT196750.15274453001847296No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA192510.14945285628389446No Hit
GAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG192380.14935193232505123No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT191790.14889389281953205No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCAC188270.1461611825493159No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC185610.14409612308375486No Hit
CTTTGAGCAGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTC184800.14346728918634716No Hit
TGAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTT183480.1424425228350161No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA182820.14193013965935058No Hit
GCAGAATTCACCAAGCGTTGGCTTGTTCACCCACTAATAGGGAACGTGAG179190.13911203219319018No Hit
GAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTG178580.13863846592477205No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162710.126317979564451No Hit
GGACAAGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT161340.12525439630587257No Hit
GTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTGCTGCCTT156030.12113204075619992No Hit
GGGAATTCCTCGTTCATGGGGAGAATTACACACCCCAATCCCTAGCACGA154160.11968028842514761No Hit
GCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCG153340.11904369114629045No Hit
CTCGTGTTCCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCATTATT151420.11755312190799072No Hit
CGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTCTGCCCTATCAACTGTC139990.10867957691123778No Hit
CTTTACTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTT139490.10829140783876387No Hit
CTCGGTTCTATTTTGTTGGTTTTCGGAACACGAGGTAATGATTAAGAGGG139260.1081128500654259No Hit
GTTATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGC138390.10743743587932134No Hit
CTCGGCTCGGGGGTCGATGAAGAGCGCAGCCAGCTGCGTGAATTAATGTG138050.1071734809100391No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATC134700.10457274812446408No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC119050.072.517347
GTTAATA95450.072.297431
TACGCTA123300.070.200669
ATATACG123500.070.197566
TATTCCC100150.068.686776
ATACGCT130050.066.552388
CTTTAAT133400.066.525621
CTTGTCT86000.065.4759141
TTGTCTC88150.063.0885542
ATTCCCG112250.061.1537557
ATATTCC114200.060.680575
AATATAC144200.060.4222685
TAATATA145150.059.8275154
TAATATT121150.056.7813453
TTTAATA158400.056.2566952
TTGAACT203500.054.3710442
GCGCCAT122900.054.0240941
GCTCGGG47000.050.4321755
TGAACTC222250.049.7056853
GCCATCC135000.049.398863
Produced by FastQC (version 0.11.5)