FastQCFastQC Report
Wed 2 Jan 2019
HCp-A-3_S15_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCp-A-3_S15_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12880985
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT738920.5736517820648033No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA600460.4661600025153356No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT481330.37367483930770823No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG473620.36768927221016096No Hit
CCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCAT421960.32758364364215936No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG403150.3129807231356919No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA388820.30185579751859043No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG365230.2835419806792726No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT361270.28046768162527946No Hit
CTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG327050.2539013903051669No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT323610.25123078708654656No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG304010.23601455944557034No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC298440.2316903559782113No Hit
GCAGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTCTGCCCT281280.2183683934109076No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTC278870.2164974184815835No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA274570.21315916445830813No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA255950.1987037481993807No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC254050.19722870572398No Hit
TCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCG250470.19444941516506697No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG235210.18260249507316403No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG232210.18027348063832074No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT231740.1799086017101953No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT225760.17526609960340767No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT218710.1697929156815259No Hit
TGAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTT217700.16900881415512867No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG214360.16641584475100313No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC211450.16415670074920513No Hit
GAACACTGTAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGGTGGGT210400.16334154569700998No Hit
CTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC207870.16137741019029214No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA207790.1613153031386963No Hit
GAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTTC204340.1586369365386265No Hit
GAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG197910.1536450822666124No Hit
CTTTGAGCAGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTC197120.15303177513210364No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA195720.15194490172917677No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC190320.14775267574645884No Hit
CTCGGCTCGGGGGTCGATGAAGAGCGCAGCCAGCTGCGTGAATTAATGTG189080.1467900164467236No Hit
GAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTG186800.14501996547624268No Hit
GCAGAATTCACCAAGCGTTGGCTTGTTCACCCACTAATAGGGAACGTGAG184590.14330425817590814No Hit
CTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCGC175710.13641037544877196No Hit
GTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTGCTGCCTT168190.13057231259876478No Hit
GGACAAGTGGCGTTTAGCCACACGAGATTGAGCAATAACAGGTCTGTGAT166440.12921372084510618No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCAC162950.12650430071923846No Hit
CTCGTGTTCCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCATTATT160400.12452463844962168No Hit
CTTTACTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTT158080.12272353395334286No Hit
GCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCG155770.12093019283851351No Hit
GGGAATTCCTCGTTCATGGGGAGAATTACACACCCCAATCCCTAGCACGA151680.11775496982567715No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATC147700.11466514400878504No Hit
GTTATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGC142750.1108222701912936No Hit
CTCGGTTCTATTTTGTTGGTTTTCGGAACACGAGGTAATGATTAAGAGGG140890.10937828124169076No Hit
ATCGGACTCGGGGAAGTATTTAGCCTTAGATGGAGTTTACCACCCACTTT139670.10843114870485449No Hit
CGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTCTGCCCTATCAACTGTC135500.10519381864042229No Hit
GACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCGT130310.1011646236681434No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA90350.082.419571
TATACGC124450.075.324767
TATTCCC100700.073.803296
ATATACG127300.073.418566
TACGCTA127850.072.924659
ATACGCT134100.069.633988
CTTGTCT85150.069.059631
CTTTAAT138850.067.730171
TAATATA142550.065.6151054
ATTCCCG114250.064.979737
TAATATT115050.064.4718863
TTGTCTC91400.064.3357852
AATATAC146750.064.0336465
ATATTCC117150.063.93515
TTTAATA149500.062.515782
TTGAACT212450.057.472932
GCTCGGG57400.055.2015045
AACTCTC239200.050.742945
GCGCCAT124350.050.3214451
TTAATAT227950.049.2075843
Produced by FastQC (version 0.11.5)