FastQCFastQC Report
Wed 2 Jan 2019
HCp-A-1_S13_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCp-A-1_S13_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042662
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA67560.33074488094457133No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTC67090.3284439618497823No Hit
GTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTGCTGCCTT60350.2954478029159988No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG59950.2934895738991571No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA57600.2819849784252118No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT51750.25334587905390127No Hit
CCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCAT49510.2423797965595874No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG44310.21692281934064472No Hit
GTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATCGGACTCGGGGA44280.21677595216438159No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT42680.2089430360970146No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT42340.20727854143269908No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT39900.19533334442996442No Hit
GAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTG39380.19278764670807017No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38030.18617862377622926No Hit
GCAGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTCTGCCCT34620.16948472140765333No Hit
CTTGATTCGGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGAT33910.16600886490275923No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC33590.16444228168928585No Hit
GCAGAATTCACCAAGCGTTGGCTTGTTCACCCACTAATAGGGAACGTGAG32350.15837177173707642No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG31870.15602189691686633No Hit
CTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC31630.15484695950676128No Hit
GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA31480.15411262362544562No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATC31200.1527418633136564No Hit
TCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCG31060.15205648315776177No Hit
GCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACC30090.14730777779192053No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA29960.146671353361447No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA29520.14451730144292105No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC29510.1444683457175No Hit
CGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTCTGCCCTATCAACTGTC28520.13962172890081667No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG28310.13859365866697476No Hit
GAACACTGTAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGGTGGGT28240.13825096858902747No Hit
CTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCGC27730.13575422659255423No Hit
GAAGTATTTAGCCTTAGATGGAGTTTACCACCCACTTTGGGCTGCATTCT27500.13462824490787023No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCAC27280.13355121894860725No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT26870.13154403420634447No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA26290.12870460213192395No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA26280.1286556464065029No Hit
GATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCGCGA25970.12713801891845053No Hit
CTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG25720.12591412578292444No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC25520.12493501127450357No Hit
CTAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAGCCCTGCACCGA24900.12189975629839885No Hit
GACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCGT24460.11974570437987292No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG24320.11906032422397833No Hit
GAAGCTCCGACCTCACGGGAAGAGCGCTTTTATTAGATCAAAACCGATCG24280.11886450132229415No Hit
GCACCATTGGCTTATCAGCCAAGCACATAAACCAAATGTCTGAACCTGCG24060.11778747536303118No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGCTTCGCCTGTACAATGGTGCATG23900.1170041837562945No Hit
GCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCGCGAATGGCT23350.11431161885813708No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT23340.11426266313271603No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCTA22490.11010142647192732No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG22400.10966082494313792No Hit
GTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCT22340.10936709059061166No Hit
GAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCTCGGGTTCTGCCTAGG22300.10917126768892747No Hit
AAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCG21780.10662556996703322No Hit
GAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTTC21730.106380791339928No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGAA21520.10535272110608607No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT21160.1035903149909285No Hit
GGGAATTCCTCGTTCATGGGGAGAATTACACACCCCAATCCCTAGCACGA21100.10329658063840223No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA21050.10305180201129703No Hit
GCCGAATCAACTAGCCCTGAAAATGGATGGCGCTAGAGCGTCGCACCTAT20930.1024643333062445No Hit
GTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGGGAGAGACCGCG20740.10153417452324466No Hit
GAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG20510.10040819283856067No Hit
CTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTG20490.10031028138771858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA7450.070.1275561
TATTCCC7500.069.586726
CTTGTCT5350.062.3902241
GCGCCAT18900.062.196471
GCCATCC19250.060.248243
TATACGC15050.060.2029887
CGCCATC19450.059.6433262
TACGCTA15900.057.4474959
ATATACG16150.056.1038446
GCTATAT4800.055.88433145
TTGTCTC6400.055.510812
CATCCAT20850.055.2772255
ATATTCC9750.055.015145
ATACGCT17000.053.723738
CTTTAAT18100.051.7158471
AATATAC18450.049.895635
TCCATTT23150.049.78417
CCATCCA25000.049.000654
ATTCCCG10750.048.5476887
TAATATA19150.048.071774
Produced by FastQC (version 0.11.5)