FastQCFastQC Report
Wed 2 Jan 2019
HC-R-neg_S22_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-R-neg_S22_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304351
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT73660.31965616349245407No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57420.24918078886419648No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC41960.1820903152340941No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG41100.17835824490279475No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGCTCGG34400.1492828132519742No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA34230.14854507841904294No Hit
GCGAACATGAGTGCCAGCTATCCTGAGGGAAACTTCAGAGGGAACCAGCT34140.14815451291925577No Hit
GTCGGATTCCCCTGGTCCGTGCCAGTTCTAAGTTGGCTGTTAGTCGCCGG32330.14029980675686995No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC32270.14003942975701184No Hit
GTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGTC32150.13951867575729565No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC32000.13886773325765042No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA31670.13743565975843092No Hit
GGAACGGCCGCGGGCTGGGCGAGACCGTGCGCAAGTTCGGTCGAGCTCGG29710.12893001109639982No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA28940.1255885062648876No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT27250.11825455410221794No Hit
GCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAACA23170.1005489181118675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCT21600.028.531162
TCTAGCT1606.8413094E-527.1820287
GTTCTTA24000.025.9739384
TATACGC12250.025.4438557
ATTCCCG5700.025.4334777
ATATACG12350.025.2378336
CCCTACT11650.024.8876937
AATATAC13000.024.5335245
TCCAGTC53800.024.5210867
CTTAGGT8300.024.452917
TCTTAGG9600.024.1618026
CTACTGA12100.023.9652399
TATTCCC6050.023.9621186
ACTCTTA8200.023.8671464
TAATATA13150.023.7024524
TTAGTTG24800.023.6751848
CCTACTG12250.023.6692188
TACCCTA12300.023.572495
ATATTCC6150.023.572495
TTGACTC57100.023.3633962
Produced by FastQC (version 0.11.5)