FastQCFastQC Report
Wed 2 Jan 2019
HC-R-neg_S22_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-R-neg_S22_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304351
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT89330.38765795662205976No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG44660.19380728022770835No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC42320.1836525772332427No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGCTCGG36780.15961110091301195No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA35470.1539262030827769No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC34720.1506714905845507No Hit
GCGAACATGAGTGCCAGCTATCCTGAGGGAAACTTCAGAGGGAACCAGCT34530.1498469634183334No Hit
GTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGTC34120.14806772058596976No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA33560.1456375352539609No Hit
GGAACGGCCGCGGGCTGGGCGAGACCGTGCGCAAGTTCGGTCGAGCTCGG30800.13366019326048853No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC30700.13322623159405836No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT29520.1281054839301825No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28720.12463379059874125No Hit
GTCGGATTCCCCTGGTCCGTGCCAGTTCTAAGTTGGCTGTTAGTCGCCGG28240.1225507745998765No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT27200.11803757326900287No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA26580.11534701093713587No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA23510.10202438777773004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC12200.040.41037
AATATAC12400.039.175545
ATATACG12850.037.803636
TAATATA12950.037.5117154
ATACGCT14200.034.7187088
TTAATAT15950.034.547353
TTTAATA14750.034.407922
TACGCTA14450.034.1180429
CTTTAAT15100.033.130241
CTACAAT4700.032.393288145
CTTATAA1606.831404E-527.1878158
ACTCTTA9250.026.6501124
GTTAATA5200.026.4912661
CCGCATA13700.024.8730892
CTTAGGT10300.023.9323167
CATTATG8550.023.742449145
CCCTACT11000.023.7275457
TTAGGTG10750.023.6049238
TTGACTC58050.023.3556442
ATTCCCG4353.6379788E-1223.3336017
Produced by FastQC (version 0.11.5)