FastQCFastQC Report
Wed 2 Jan 2019
HC-R-3_S3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-R-3_S3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12832133
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT332360.2590060436561872No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA326960.2547978578463923No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA312050.2431785892493477No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC293690.22887075749604527No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA284730.22188828622645979No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTT276350.21535780528459297No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC274370.21381480382100154No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC261490.2037775013709724No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC256720.2000602705723203No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT256580.199951169458733No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA245540.191347767358708No Hit
GTTTATGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACT236320.18416267973531758No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG227240.17708669322551443No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA210910.16436082761922746No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT208550.16252169456161342No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA208260.16229569939775404No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG206330.16079166261758665No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG203870.15887460019312455No Hit
GGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCCAGAC193770.15100373414147125No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCT191280.14906329290695478No Hit
GGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAG185590.14462911193330055No Hit
GTCAGTCCCTCTTAATCATTACCTCGTGTTCCGAAAACCAACAAAATAGA183330.1428679082425346No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA181170.14118463391861663No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG178680.13924419268410015No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG178040.1387454447362726No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGAC177180.13807525218137937No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA172770.13463856710338024No Hit
GTGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGAC165700.12912896086722292No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG163850.12768726758053398No Hit
GGGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCCAGA162650.12675211517835733No Hit
GTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAGGACGGAAAC160780.12529483601829874No Hit
GCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACC158380.12342453121394548No Hit
GAAACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGCTTCGCCT156470.1219360803071477No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGCTGGA155950.1215308475995378No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA153900.11993329557915273No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA147830.11520298301147597No Hit
GTGAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCA145650.11350412281418841No Hit
GCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCAC142250.11085452434135463No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA141820.11051942806390799No Hit
GCTGATTCCACCAGGCCCGTTCCCCTGGCTGTGGTTTCGCTAGATAGTAG141360.11016095297640696No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA141330.11013757416635254No Hit
GGGAGCTTCGCGGCGCAATTGAAATACCACTACTCCCATCGTTTCTTTAC140530.10951413923156812No Hit
GGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGC140090.10917125001743669No Hit
GTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGTGGCTGCTCTACTAG139420.10864912325955474No Hit
GAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAG139390.10862574444950032No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG137610.107238601719605No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTC136270.1061943482038411No Hit
CTCCGCTTAACTGAATAAGTAAAGAAACGATGGGAGTAGTGGTATTTCAA135420.10553194858563264No Hit
GTTGATTCCTTCAGTGTAGCGCGCGTGCGGCCCCGAACATCTAAGGGCAT134460.10478382666389134No Hit
CCCTTCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC131240.10227450105138405No Hit
TTTTACTCTACGCGAGGTTTCCGTCCTCGCTGAGCTCACCTTAGGACACC130400.10161989436986041No Hit
CCAGAACTTGCCAAGGGGCTTGCGCCCCGCTCTCCGCTTAACTGAATAAG129960.10127700515572897No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT129730.10109776761197846No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTAG48950.072.725132
CAGTAGT50000.071.46983
CTAACAT78750.065.881231
AGTAGTC56700.062.640764
TAACATG84350.061.54372
ACATGTG89100.058.4104774
AACATGT89950.058.1810723
TGTGCGC90150.057.4891667
ATGTGCG94200.055.5559126
TAGTCAT65150.054.071186
CATGTGC97600.053.620565
GTAGTCA65950.053.3053675
GCTCGGG24650.048.5189065
TCGAAAG46850.047.9742052
CTTGTTA15250.046.6296841
AGGGAAT33100.046.2552151
GTCATAT77850.045.3453678
TCATATG78500.045.158279
TGCACCC17300.043.165825145
CGCCCTT14450.042.683231
Produced by FastQC (version 0.11.5)