FastQCFastQC Report
Wed 2 Jan 2019
HC-R-3_S3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-R-3_S3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12832133
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA364210.2838265469972919No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT350320.27300215794209737No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA329270.2565980262205823No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTT321610.25062863672002156No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA312470.24350589259010952No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC307790.23985879822162065No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC273750.2133316417465436No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC272080.21203022132018112No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC268770.2094507592775106No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA266700.20783762138375592No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT266040.20732328756255877No Hit
GTTTATGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACT258860.2017279590228686No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG244000.190147655109248No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT235820.18377303290107733No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG224000.17456178173963752No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA221020.17223948660756555No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG205320.16000457601242132No Hit
GGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCCAGAC200370.15614707235344272No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA197690.1540585653219149No Hit
GTCAGTCCCTCTTAATCATTACCTCGTGTTCCGAAAACCAACAAAATAGA190960.148813918933041No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGAC187340.1459928758531415No Hit
GGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAG186890.14564219370232526No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG184970.14414594985884263No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA176190.13730375144958362No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG169050.13173959465663268No Hit
GTGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGAC168830.13156815004956698No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCT166390.1296666734984745No Hit
GTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAGGACGGAAAC165560.12901985975363567No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA165490.12896530919684202No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA163390.12732879249303292No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG161330.12572344753596304No Hit
GAAACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGCTTCGCCT160720.1252480783981899No Hit
GGGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCCAGA159260.12411030964220836No Hit
GCTGATTCCACCAGGCCCGTTCCCCTGGCTGTGGTTTCGCTAGATAGTAG157760.12294136913948758No Hit
TTTTACTCTACGCGAGGTTTCCGTCCTCGCTGAGCTCACCTTAGGACACC156540.12199063086394132No Hit
GCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCAC153890.11992550264246793No Hit
GTGAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCA153160.11935661826447716No Hit
GTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGTGGCTGCTCTACTAG151870.11835132943213728No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGCTGGA151700.1182188495084956No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA150260.11709666662588364No Hit
GTTGATTCCTTCAGTGTAGCGCGCGTGCGGCCCCGAACATCTAAGGGCAT149100.11619268597044623No Hit
CCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTTT147190.11470423506364844No Hit
CCAGAACTTGCCAAGGGGCTTGCGCCCCGCTCTCCGCTTAACTGAATAAG146110.11386259790168946No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTC144840.11287289494271918No Hit
GGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGC144450.11256897041201179No Hit
GAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAG144090.11228842469135879No Hit
GCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACC142410.11097921132831151No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA141110.10996612955928683No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA140220.10927255819433916No Hit
CCCTTCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC139320.1085711938927067No Hit
GACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCGT134870.10510333706796836No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT134170.10455783150003198No Hit
ATCGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGT131880.10277324899921159No Hit
GGGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGA130750.10189264715382859No Hit
CTCCGCTTAACTGAATAAGTAAAGAAACGATGGGAGTAGTGGTATTTCAA129150.10064577728425975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACAT77850.070.6970441
CCAGTAG44900.069.765162
TAACATG82650.066.851832
CAGTAGT48200.065.1391453
AACATGT89100.062.5820543
ACATGTG89750.061.9672364
TGTGCGC92350.060.13457
ATGTGCG93350.060.043556
CATGTGC95900.058.825035
AGTAGTC54000.058.0084344
GCTCGGG32800.054.6039775
TCGAAAG44950.054.5240552
CTTGTTA14100.050.9136471
TAGTCAT61500.050.5805246
TGCACCC17350.048.891506145
GTAGTCA64250.048.528465
AGGGAAT32000.047.813511
TCGGGGG42450.042.8674667
CGCCCTT13700.041.814281
GTCATAT74900.041.5246128
Produced by FastQC (version 0.11.5)