FastQCFastQC Report
Wed 2 Jan 2019
HC-R-2_S2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-R-2_S2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15698572
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC565020.35991808681706844No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA468100.2981799873262358No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC452620.2883192178243983No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC419600.26728545755626687No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT407500.25957775012912004No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC373180.23771588906303073No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT350300.22314131501897116No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA316660.2017126143702752No Hit
GTCAGTCCCTCTTAATCATTACCTCGTGTTCCGAAAACCAACAAAATAGA298730.1902911933645939No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG288190.18357720689499657No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT274050.17457001821566956No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG272030.1732832769757657No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTT269720.17181180555785583No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG261500.16657566051230646No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG259980.1656074195793095No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA249680.15904631325702745No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG248670.15840294263707552No Hit
GGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCCAGAC247350.15756210182684133No Hit
GTTTATGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACT245450.15635180066059512No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA238320.15180998628410278No Hit
GGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAG229480.14617890085798887No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT219440.1397834146952984No Hit
GTCAGAATCTCGCCCAGATCTACAACGATACTCTTTATGCACTTCGGCCT218470.13916552409989902No Hit
CTCGGTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTG214880.13687869189630752No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG214660.13673855176126848No Hit
CTCGGCTCGGGGGTCGATGAAGAGCGCAGCCAGCTGCGTGAATTAATGTG208050.13252797770395933No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA207290.13204385723746084No Hit
GTGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGAC202250.12883337414383933No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTC197510.12581399123436196No Hit
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT193890.12350804901235604No Hit
GTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAGGACGGAAAC192490.12261624815301671No Hit
GACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCGT189880.12095367655096273No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA189070.12043770605377356No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC188050.1197879654276835No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG186340.11869869437806191No Hit
GAAAGGCTTAGTCTCAATAGATCGCAGTGTGGTGGCTGCTCTACTAGGTA185040.11787059358010397No Hit
GTTAATTCCGATAACGAACGAGACTCTAACCTACTAAATAGTTCACCGAT183580.11694057268393583No Hit
GGGAGCTTCGCGGCGCAATTGAAATACCACTACTCCCATCGTTTCTTTAC183140.11666029241385777No Hit
GAAACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGCTTCGCCT180720.1151187509284284No Hit
TTTTACTCTACGCGAGGTTTCCGTCCTCGCTGAGCTCACCTTAGGACACC180470.11495950077497494No Hit
GAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAG180320.11486395068290288No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCT179490.11433524017343744No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGAC175760.111959227883912No Hit
CTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCGC175390.11172353765680088No Hit
GGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGC173830.11072981669925136No Hit
GTGAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCA172010.10957047558211026No Hit
CCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTTT171810.10944307545934752No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA171770.10941759543479496No Hit
CTTCAGTGTAGCGCGCGTGCGGCCCCGAACATCTAAGGGCATCACAGACC167810.10689507300409234No Hit
GAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAG167020.10639184251917946No Hit
GGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCCAGACGGCCTACAGCG164770.10495859113809841No Hit
GCTGATTCCACCAGGCCCGTTCCCCTGGCTGTGGTTTCGCTAGATAGTAG164250.10462735081891525No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA163580.10420056040766001No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAG162460.10348711972018856No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161010.10256346883015857No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT160530.10225770853552794No Hit
GTACGCTCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGT158740.10111747743680125No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA158700.1010919974122487No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT157870.10056328690278325No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA102950.061.182171
TATTCCC110100.056.817986
TAATATT112250.055.794823
TCATATG55900.055.775682
GTCATAT57550.054.723761
ATATTCC113950.054.5802235
ATTCCCG114150.054.4847687
CATATGC58850.052.8417663
GCTCGGG62000.051.208825
AGGCTTA60050.048.405544
AAGGCTT61900.046.959153
TCGAAAG38750.046.5924842
TATACGC163450.045.5458957
TACGCTA166250.044.738919
ATACGCT166250.044.6487128
ATATACG167700.044.4347156
AATATAC173450.043.379595
TAATATA174050.043.0219464
CTAACAT59150.042.9382971
CTTTAAT176900.042.8256721
Produced by FastQC (version 0.11.5)