FastQCFastQC Report
Wed 2 Jan 2019
HC-R-2_S2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-R-2_S2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15698572
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC619950.39490853053386005No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA521680.33231048021437876No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC437910.27894893879519744No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC436050.2777641176535038No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT434760.276942386861684No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC381030.24271634388146898No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT372970.2375821189341298No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA318390.20281462543217305No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTT315380.20089725358459357No Hit
GTCAGTCCCTCTTAATCATTACCTCGTGTTCCGAAAACCAACAAAATAGA303890.1935781165318731No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG283410.18053234396096662No Hit
CTCGGCTCGGGGGTCGATGAAGAGCGCAGCCAGCTGCGTGAATTAATGTG274990.1751687987926545No Hit
GTTTATGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACT273110.1739712376386846No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT271790.17313039682845038No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG268550.1710665148396937No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG266820.16996450377779584No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA263650.1679452118320061No Hit
GGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCCAGAC257550.164059508087742No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT249970.15923104343503344No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA248390.15822458246520765No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG248190.1580971823424449No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG247470.15763854190049897No Hit
GGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAG232280.1479625025766675No Hit
TTTTACTCTACGCGAGGTTTCCGTCCTCGCTGAGCTCACCTTAGGACACC225540.1436691184395625No Hit
GTCAGAATCTCGCCCAGATCTACAACGATACTCTTTATGCACTTCGGCCT221430.14105104591678785No Hit
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT219830.14003184493468576No Hit
CTCGGTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTG219280.13968149459708817No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG217520.13856037351677591No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTC207800.13236872755050588No Hit
GTGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGAC206630.13162343683234373No Hit
GTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAGGACGGAAAC206150.13131767653771312No Hit
GTTAATTCCGATAACGAACGAGACTCTAACCTACTAAATAGTTCACCGAT202750.12915187445074622No Hit
CCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTTT202290.12885885416839188No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC199260.12692874230853607No Hit
GAAAGGCTTAGTCTCAATAGATCGCAGTGTGGTGGCTGCTCTACTAGGTA196030.12487123032591754No Hit
CTTCAGTGTAGCGCGCGTGCGGCCCCGAACATCTAAGGGCATCACAGACC194950.12418326966299865No Hit
GACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCGT194800.12408771957092658No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGAC193670.123367908877317No Hit
GAAACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGCTTCGCCT187950.11972426536630211No Hit
GTGAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCA183700.11701701275759349No Hit
GAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAG181860.11584493162817612No Hit
GCTGATTCCACCAGGCCCGTTCCCCTGGCTGTGGTTTCGCTAGATAGTAG179470.11432250016116116No Hit
GAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAG176890.11267903857752157No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA176230.11225861817240447No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA172420.10983164583377393No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA172380.10980616580922138No Hit
GGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGC168390.10726453336010434No Hit
GTACGCTCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGT166640.10614978228593022No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA166580.10611156224910138No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAG166380.10598416212633861No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG166230.10588861203426655No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA165330.10531531148183415No Hit
GGGAGCTTCGCGGCGCAATTGAAATACCACTACTCCCATCGTTTCTTTAC163150.10392665014372009No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCT161730.1030221092721045No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT161570.10292018917389428No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG161000.10255709882402042No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT160670.10234688862146187No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA160510.10224496852325167No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA159610.10167166797081925No Hit
CTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCGC159550.10163344793399044No Hit
GCGGATTGCTAGTTGGCATCGTTTATGGTCAGAACTACGACGGTATCTGA157400.10026389661429078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA105150.064.342181
TAATATT112450.060.4857333
GCTCGGG73700.060.0166745
TATTCCC114150.059.5849386
ATTCCCG117500.057.9382487
ATATTCC118700.057.178765
GTCATAT57000.056.8664471
TCATATG57050.056.433132
CATATGC60550.053.5307043
AGGCTTA59800.051.655674
AAGGCTT61500.050.3456953
CTAACAT57100.049.1471441
TATACGC174550.048.5548137
TACGCTA176650.048.0183379
ATATACG176750.047.95846
ATACGCT177750.047.680398
TCGAAAG33750.047.266752
AATATAC181100.046.9265635
TAATATA179500.046.860094
AAAGGCT66750.046.8201522
Produced by FastQC (version 0.11.5)