FastQCFastQC Report
Wed 2 Jan 2019
HC-R-1_S1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-R-1_S1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3169664
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGCATAGGT146710.4628566308605581No Hit
CTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAGCATGTT117250.36991302548156524No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA88800.28015587772079314No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC80320.2534022533618705No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT73820.23289534789807373No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA69780.2201495174251908No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68430.21589039090578688No Hit
GTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATCGGACTCGGGGA68030.21462842749263014No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC67090.21166281347171184No Hit
CTTGATTCGGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGAT66490.20976986835197675No Hit
GTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGC66220.20891804304809594No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG64480.20342850220086423No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT63320.1997688083027097No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC58570.18478299277147356No Hit
GTGAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCA52230.16478087267293948No Hit
GTTAATTCCGATAACGAACGAGACTCTAACCTACTAAATAGTTCACCGAT52160.16456002907563705No Hit
GCGCAATTGAAATACCACTACTCCCATCGTTTCTTTACTTATTCAGTTAA52110.16440228364899245No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG50550.1594806263376812No Hit
CGAGACTCTAACCTACTAAATAGTTCACCGATCTATTGCTGTCGGTGTAA50160.15825021200985342No Hit
GTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGG49630.15657811048742074No Hit
GCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACC47830.15089927512821547No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA46160.14563057787828615No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCTA45470.1434536909905908No Hit
GGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGC44160.13932076081250253No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATCAAG43760.1380587973993458No Hit
CTCGGTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTG42320.13351572911198156No Hit
GGTACATCTGTCAAACGGTAACGCAGGTGTCCTAAGGTGAGCTCAGCGAG41990.13247460929612728No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT41470.13083405685902355No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT41350.13045546783507653No Hit
GCGGATTGCTAGTTGGCATCGTTTATGGTCAGAACTACGACGGTATCTGA41310.13032927149376086No Hit
GGTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTGTTG40790.1286887190566571No Hit
CTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG40450.1276160501554739No Hit
GTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGTC39350.12414565076929289No Hit
GAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCATTTGCCAAG39110.12338847272139887No Hit
GAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCTCGGGTTCTGCCTAGG38930.12282058918547833No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC38520.1215270766869927No Hit
GACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCGT37640.11875075717804788No Hit
GTTAGAGGTGAAATTCTTGGATCCTCGCCAGACGGCCTACAGCGAAAGCA37580.1185614626660744No Hit
GTCGGATTCCCCTGGTCCGTGCCAGTTCTAAGTTGGCTGTTAGTCGCCGG37550.11846681541008762No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG37510.11834061906877197No Hit
GTGAAGGCAGCCTCGGGTTCTGCCTAGGTGGGATCCTCGGTCCGTCGCGG37360.11786738278883818No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATC37150.11720485199693091No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC37150.11720485199693091No Hit
GAACACTGTAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGGTGGGT36820.1161637321810766No Hit
GATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGGGAGAATTACACAC35510.11203080200298833No Hit
CTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCGC34440.10865504987279408No Hit
GTTCGATTAGTCTTTCGCCCCTATGCTCAAGTTTGACGATCGATTTGCAC34050.10742463554496628No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGCTCGG33630.10609957396115172No Hit
AGAAGACCCTGTTGAGCTTGACTCCAGTCCGACTTTGTGAAGAGACATGA33520.10575253402253361No Hit
CTCGGTTCTATTTTGTTGGTTTTCGGAACACGAGGTAATGATTAAGAGGG33210.10477451237733716No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTC32910.1038280398174696No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG32900.1037964907321407No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA32750.10332325445220691No Hit
ATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCTCGG32560.10272382183095748No Hit
GGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCA32240.10171425110043211No Hit
GTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCT32210.10161960384444534No Hit
CTTCAGTGTAGCGCGCGTGCGGCCCCGAACATCTAAGGGCATCACAGACC32190.1015565056737875No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT32160.10146185841780075No Hit
GATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCGCGA31940.10076777854056455No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA31890.10061003311391996No Hit
GTTAAGGTGCCTAAGGCACGACGCTCATGAGATACCATAAAAGGTGTTGG31740.10013679683398619No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCGACTAGAGGCTGTTCACCTT31710.10004214957799944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCC8200.062.7651756
GCTATAT14850.061.511948145
ATTCCCG8500.060.549947
ATATTCC8950.057.5055245
GTTAATA9000.057.240231
TATAGTT7850.053.564087145
TTGTCTC6700.051.9482462
TATACGC25050.051.2200247
CTTGTCT6700.050.898921
ATATACG25850.049.634886
TAATATT10750.049.2253343
CTTTAAT26350.049.0145381
ATACGCT26350.048.695358
AATATAC26500.048.417425
TACGCTA27350.046.917869
TAATATA28800.044.550754
TTTAATA30300.042.597282
CCGTATA7400.042.126183145
CTTAGTC6100.041.631661
TAGTGTT4500.040.27568145
Produced by FastQC (version 0.11.5)