FastQCFastQC Report
Wed 2 Jan 2019
HC-E-3_S9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-E-3_S9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15690866
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTC711950.4537353132707908No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT704910.44924862655764186No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA634970.404674923614796No Hit
GTTTGAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCAC519750.3312436674941969No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC502810.3204475775906824No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG482690.30762483090480797No Hit
GGAGAATTACACACCCCAATCCCTAGCACGAAGGAGGTTCAACGGGTTAC480630.306311965190449No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG474160.3021885471458363No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC464120.2957899200719705No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTC408270.26019596368995823No Hit
ATTTTACACTTCTACATTGACATGGGATGGATCTCGCTTCGACCGACACC366200.23338418669817204No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG365630.23302091802963584No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT347980.22177233557408496No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT347100.22121149973494134No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG340640.21709445482486436No Hit
ATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTC335360.2137294397900027No Hit
GTAGATCTGGGCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCA334100.21292642483850158No Hit
GTTATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGC330390.2105619919257484No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT316910.20197100657159392No Hit
GGCGAAGCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTTTCG284760.18148137903924488No Hit
GGGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC282850.18026411034292178No Hit
TGAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCG282780.18021949840117174No Hit
GTTCGATTAGTCTTTCGCCCCTATGCTCAAGTTTGACGATCGATTTGCAC273740.17445818478087824No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA260180.16581621435043803No Hit
TGATTTTACACTTCTACATTGACATGGGATGGATCTCGCTTCGACCGACA258880.16498770686079403No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC254280.1620560649743615No Hit
GAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGG249720.1591499156260719No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC248130.15813658723489193No Hit
GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATA242180.1543445721861368No Hit
GTACGCTCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGT238030.15169972135381182No Hit
ATCGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGT233340.1487107212565578No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCAC228950.14591291519537544No Hit
CACCATTGTACAGGCGAAGCGCCTGCTTTGAACACTCTAATTTTTTCAAA224160.14286018375276419No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT223880.14268173598576395No Hit
GACCGACTGACCCATGTCCAACTGCTGTTCACATGGAACCCTTCTCCACT219600.13995403440447454No Hit
GCCGGCGCCCGCGATGCGGGTCCGCCGACACTTATCGTTGCCGCCCAAGG213940.13634684025725538No Hit
CGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCG208730.13302643716414378No Hit
CGACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCC198290.12637288470884908No Hit
CTTGCCGGCGCCCGCGATGCGGGTCCGCCGACACTTATCGTTGCCGCCCA197570.12591401902227706No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA196300.12510463093624022No Hit
GGGCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAG193540.1233456458043807No Hit
GTACAGGCGAAGCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAG190020.12110230244780627No Hit
GGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGT189640.1208601233354488No Hit
GTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGG187360.11940704866130397No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC185810.11841921280826692No Hit
GAATTACACACCCCAATCCCTAGCACGAAGGAGGTTCAACGGGTTACCCG184390.11751422770419427No Hit
CCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGCCCGAAGCTTC177210.11293831710754525No Hit
AGGGAATCGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGC175980.11215442155965133No Hit
GGGAGAATTACACACCCCAATCCCTAGCACGAAGGAGGTTCAACGGGTTA172440.10989833193400542No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT170180.10845800352893206No Hit
CCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCATTATTCCATGCAC169830.10823494382018176No Hit
GGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGGCCGTCACCT169400.10796089903514568No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167520.10676274974242977No Hit
GCTTAACTGAATAAGTAAAGAAACGATGGGAGTAGTGGTATTTCAATTGC161680.1030408391735676No Hit
GTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCGCTAG157060.10009645101806362No Hit
GTCGGATTCCCCTGGTCCGTGCCAGTTCTAAGTTGGCTGTTAGTCGCCGG156990.10005183907631357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATT30350.067.426331
CCAGTAG59900.065.0069352
CAGTAGT63450.061.463733
AGGGAAT49850.061.4308241
CTAACAT166550.061.1732521
CGCTATT129650.060.337221
TAACATG172050.058.8359762
ATGTGCG181250.056.1100966
ACATGTG181400.055.944174
AACATGT184900.054.9641273
CATGTGC186550.054.282835
TGTGCGC188300.054.0866557
AGTAGTC72850.053.631924
CTATTGG147550.052.9108543
GCTATTG148450.052.558662
CGCATAA11250.050.958151
TATTGGA155050.050.584754
GTACCTT19650.046.1117484
TTGGAGC170600.045.5913056
CTCGTTT253950.045.0061041
Produced by FastQC (version 0.11.5)