FastQCFastQC Report
Wed 2 Jan 2019
HC-E-3_S9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-E-3_S9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15690866
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT752800.4797695678492188No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTC709890.4524224475564319No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA687930.4384270441159844No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC563830.3593364445276634No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG502980.32055592087778967No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG496520.3164388759677127No Hit
GTTTGAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCAC492650.31397247290238794No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC481640.30695565177855705No Hit
GGAGAATTACACACCCCAATCCCTAGCACGAAGGAGGTTCAACGGGTTAC462820.29496141258232655No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTC427020.27214559094443863No Hit
ATTTTACACTTCTACATTGACATGGGATGGATCTCGCTTCGACCGACACC386750.2464809781690826No Hit
ATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTC359600.22917791790459494No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG348260.2219507833410852No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT346030.220529574339619No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG345850.22041485791797596No Hit
GTAGATCTGGGCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCA339930.216641962272828No Hit
GTTATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGC333510.21255040990089394No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT326970.20838237991453118No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT324220.20662976791720736No Hit
TGAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCG312400.1990967228959829No Hit
GGCGAAGCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTTTCG286600.1826540357938179No Hit
TGATTTTACACTTCTACATTGACATGGGATGGATCTCGCTTCGACCGACA284420.1812646924650303No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC277510.1768608565008458No Hit
GGGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC277360.17676525948280994No Hit
GTTCGATTAGTCTTTCGCCCCTATGCTCAAGTTTGACGATCGATTTGCAC268240.17095296078623068No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA265280.16906651296365668No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC261890.16690602035604665No Hit
GTACGCTCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGT252700.1610491097177173No Hit
GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATA248550.1584042588853923No Hit
CACCATTGTACAGGCGAAGCGCCTGCTTTGAACACTCTAATTTTTTCAAA248040.15807922902407046No Hit
ATCGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGT241320.15379648261606466No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT236420.15067364669356043No Hit
GAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGG220690.14064870606886837No Hit
CTTGCCGGCGCCCGCGATGCGGGTCCGCCGACACTTATCGTTGCCGCCCA213580.13611740741396935No Hit
CCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGCCCGAAGCTTC209000.1331985117966083No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCAC204610.13040070573542595No Hit
GCCGGCGCCCGCGATGCGGGTCCGCCGACACTTATCGTTGCCGCCCAAGG204180.13012666095038986No Hit
GTACAGGCGAAGCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAG203680.12980800422360372No Hit
GACCGACTGACCCATGTCCAACTGCTGTTCACATGGAACCCTTCTCCACT201060.12813824297524432No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA200620.1278578250556725No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC199850.12736709369642185No Hit
CGACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCC195070.12432073538834632No Hit
GAATTACACACCCCAATCCCTAGCACGAAGGAGGTTCAACGGGTTACCCG191480.12203278009002182No Hit
CGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCG191360.12195630247559314No Hit
CCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCATTATTCCATGCAC190350.12131261588748511No Hit
AGGGAATCGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGC186280.11871875013144591No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG182980.11661561573465735No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT182450.11627783960426402No Hit
GGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGT182150.11608664556819234No Hit
ATTACACACCCCAATCCCTAGCACGAAGGAGGTTCAACGGGTTACCCGAC172650.1100321677592556No Hit
GTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCGCTAG169450.10799276470782428No Hit
GAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACA162080.10329576455499652No Hit
GCTTAACTGAATAAGTAAAGAAACGATGGGAGTAGTGGTATTTCAATTGC160850.10251186900710262No Hit
TTTGAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACC158150.10079112268245743No Hit
GGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGGCCGTCACCT157880.1006190480499929No Hit
GGGCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAG157190.10017930176702804No Hit
TGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGC157180.1001729286324923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATT27850.065.3502351
AGGGAAT46700.063.349321
CCAGTAG64400.062.8250922
CAGTAGT65000.062.5802153
CTAACAT178950.061.590051
CGCTATT137350.060.711061
TAACATG184800.059.167632
ACATGTG192700.056.5541954
ATGTGCG192800.056.3744356
AACATGT198600.055.0566373
TGTGCGC200150.054.514817
CATGTGC201850.053.918715
GCTATTG156900.053.3757322
AGTAGTC76200.053.1917654
CTATTGG156950.052.9894833
TATTGGA164650.050.6434974
TTGGAGC179600.046.4279066
CTCGTTT248250.046.0618171
GTTAATA252750.045.958941
CTCGATG29500.043.500668145
Produced by FastQC (version 0.11.5)