FastQCFastQC Report
Wed 2 Jan 2019
HC-E-2_S8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-E-2_S8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9821205
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT890660.9068744619422973No Hit
ATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTC653210.665101685587461No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG372990.3797802815438635No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC344350.3506188904518336No Hit
GGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGT307100.3126907543422625No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG300900.3063778833656359No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG298440.30387309907490984No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT274340.27933435866576456No Hit
GTTCGATTAGTCTTTCGCCCCTATGCTCAAGTTTGACGATCGATTTGCAC251130.255701820703264No Hit
GGAGAATTACACACCCCAATCCCTAGCACGAAGGAGGTTCAACGGGTTAC246920.25141517766913535No Hit
ATTTTACACTTCTACATTGACATGGGATGGATCTCGCTTCGACCGACACC231070.23527662847888825No Hit
GAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGG229090.2332605825863527No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC226210.23032815219721No Hit
GGCGAAGCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTTTCG217890.2218566866285756No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC202970.2066650680848226No Hit
GCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGC198480.20209332765174945No Hit
GTTTGAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCAC196010.1995783613110611No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT193820.1973484923693172No Hit
GTCACAATGTCTCCCTCGGATTTTCAAGGGACGACGGAGGTGCACCGGAC192070.19556663362591456No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG184130.1874820859558476No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA183070.18640278865984367No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG182650.18597514256142703No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA181010.18430528636760968No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTC173770.1769334821949038No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT173160.1763123771472034No Hit
GGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCTGGCACGA168460.17152681366492198No Hit
GTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCTGGCACGAAAGT164430.16742344753011468No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT162260.1652139426882954No Hit
ATTACACACCCCAATCCCTAGCACGAAGGAGGTTCAACGGGTTACCCGAC160050.16296370964662688No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCAC150060.15279184173428823No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT145870.1485255628000841No Hit
GACCGACTGACCCATGTCCAACTGCTGTTCACATGGAACCCTTCTCCACT145320.14796555005215756No Hit
GGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCA131540.13393468520410684No Hit
GAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCC130290.1326619289588192No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT129200.1315520855129284No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTC128700.13104298301481335No Hit
GGGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC128530.13086988816545425No Hit
GTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCGCTAG122700.12493375303743279No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACA122570.12480138638792286No Hit
GAATTACACACCCCAATCCCTAGCACGAAGGAGGTTCAACGGGTTACCCG121400.12361008654233364No Hit
AATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGG119400.12157367654987346No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG116590.11871252051046689No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG113750.11582081832117341No Hit
GTAGATCTGGGCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCA109380.1113712624876479No Hit
GGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGGCCGTCACCT108630.11060760874047533No Hit
ATCGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGT107960.10992541139300117No Hit
CCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGCCCGAAGCTTC107770.10973195244371745No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTT107590.10954867554439603No Hit
AAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCG106100.10803155010001318No Hit
GTCGATGGTACGTGCTATGCCTACCATGGTTGTAACGGGTAACGGGGAAT105120.1070337092037077No Hit
ACGAAAGTCACAATGTCTCCCTCGGATTTTCAAGGGACGACGGAGGTGCA104850.10675879385472556No Hit
GTTATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGC104560.10646351440581883No Hit
ATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTA102500.10436601211358484No Hit
GTCGGATTCCCCTGGTCCGTGCCAGTTCTAAGTTGGCTGTTAGTCGCCGG101230.10307289176837262No Hit
GATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCGCGA100040.10186122782285881No Hit
GTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCT99600.10141321762451756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA172500.077.4375461
TAATATT187450.071.1907963
TATTCCC190850.069.88496
ATATTCC196600.067.656625
ATTCCCG200150.066.492837
GTCATAT48400.063.2636071
AATATTC213200.062.558474
ACCGACT39200.062.5164762
TTAATAT215200.062.0599252
CATATGC48150.060.2170143
TCATATG48250.060.10722
TCCCGAA251500.053.1531879
TTCCCGA252600.052.9165958
ATATGCT58100.050.0292244
CGACTGA49150.049.553154
CTTGTCT125500.045.385051
TTGTCTC125650.044.9509472
ATTTGCT246200.043.7943041
TTGCTAC244650.043.6132133
TGCTACT248000.043.0240864
Produced by FastQC (version 0.11.5)