FastQCFastQC Report
Wed 2 Jan 2019
HC-E-2_S8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-E-2_S8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9821205
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT994251.012350317501773No Hit
ATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTC716330.7293707849495046No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG368960.3756769154090562No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC357190.363692642603428No Hit
GGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGT327980.3339508746635469No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG323710.32960313932964436No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG316280.3220378762076548No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT268960.27385641578604664No Hit
GGAGAATTACACACCCCAATCCCTAGCACGAAGGAGGTTCAACGGGTTAC257000.2616786840311347No Hit
GAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGG247360.2518631878674766No Hit
GTTCGATTAGTCTTTCGCCCCTATGCTCAAGTTTGACGATCGATTTGCAC246000.2504784290726036No Hit
ATTTTACACTTCTACATTGACATGGGATGGATCTCGCTTCGACCGACACC244880.24933803947682592No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC233440.23768977431995358No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC230770.23497116698001927No Hit
GGCGAAGCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTTTCG220240.22424946836971635No Hit
GCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGC219160.22314980697378783No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG211340.2151874439032685No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT199460.20309116854805492No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA198480.20209332765174945No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA196370.19994491510970394No Hit
GTCACAATGTCTCCCTCGGATTTTCAAGGGACGACGGAGGTGCACCGGAC194160.19769468206803542No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTC187160.19056724709442477No Hit
GTTTGAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCAC184140.18749226800580987No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG182400.1857205913123695No Hit
ATTACACACCCCAATCCCTAGCACGAAGGAGGTTCAACGGGTTACCCGAC174860.1780433256407946No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT170610.1737159544068167No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT168230.17129262651578905No Hit
GTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCTGGCACGAAAGT158710.16159931495167854No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT149230.15194673158741723No Hit
GGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCTGGCACGA148830.1515394495889252No Hit
GAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCC142250.14483966071373117No Hit
AATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGG137810.14031883053046953No Hit
GACCGACTGACCCATGTCCAACTGCTGTTCACATGGAACCCTTCTCCACT135290.1377529539399697No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT134150.13659220024426738No Hit
GTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCGCTAG132730.13514634914962065No Hit
GAATTACACACCCCAATCCCTAGCACGAAGGAGGTTCAACGGGTTACCCG130190.1325601084591962No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCAC130080.13244810590961087No Hit
GGGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC129050.1313993547634939No Hit
CCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGCCCGAAGCTTC128490.13082915996560504No Hit
GGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCA128130.1304626061669622No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTC123100.12534103503592484No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTT120580.12277515844542497No Hit
ATCGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGT120480.12267333794580197No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACA117190.11932344350820494No Hit
GTAGATCTGGGCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCA112820.11487388767467943No Hit
ATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTA112120.11416114417731836No Hit
GTCGATGGTACGTGCTATGCCTACCATGGTTGTAACGGGTAACGGGGAAT111220.11324475968071128No Hit
AAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCG110510.1125218341333879No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG109820.11181927268598914No Hit
GTTATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGC109610.11160544963678082No Hit
ATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGG108260.11023087289187018No Hit
GAAAGTCACAATGTCTCCCTCGGATTTTCAAGGGACGACGGAGGTGCACC107680.10964031399405673No Hit
GATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCGCGA105480.10740026300235053No Hit
TGAATATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCG102890.10476311206211457No Hit
GCAGAATTCACCAAGCGTTGGCTTGTTCACCCACTAATAGGGAACGTGAG102260.10412164291448962No Hit
ACGAAAGTCACAATGTCTCCCTCGGATTTTCAAGGGACGACGGAGGTGCA102060.1039180019152436No Hit
GTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCT101690.10354126606663845No Hit
GCGAACATGAGTGCCAGCTATCCTGAGGGAAACTTCAGAGGGAACCAGCT99210.10101611767598784No Hit
CGAAGCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTTTCGGC98500.10029319212866444No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA182400.083.918741
TAATATT195500.078.478593
TATTCCC205850.074.286186
ATATTCC210550.072.800115
ATTCCCG216550.070.641537
TTAATAT222350.068.9696042
AATATTC228150.067.152374
GTCATAT46100.060.555751
CATATGC46800.059.0284583
ACCGACT36450.058.8814772
TCATATG47300.058.558072
TCCCGAA268500.057.3513839
TTCCCGA269750.057.0856178
TGCGACG186450.052.843937145
ATATGCT58500.047.8424844
TTGCTAC241500.046.2966463
ATTTGCT246750.045.8125571
CGACTGA47500.045.3362054
TGCTACT246350.045.2674524
TTGTCTC128600.044.4293332
Produced by FastQC (version 0.11.5)