FastQCFastQC Report
Wed 2 Jan 2019
HC-E-1_S7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-E-1_S7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825811
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTC80730.4421596758919735No Hit
GCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGC68010.3724920049227439No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT54680.29948335287715977No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATT52960.2900628816454715No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT50120.27450815007686996No Hit
GTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTGCTGCCTT44660.24460363093441764No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG41960.22981568190793022No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC41730.22855596773159984No Hit
GTTCGATTAGTCTTTCGCCCCTATGCTCAAGTTTGACGATCGATTTGCAC41570.2276796448263265No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCAC40950.22428389356839237No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT40780.22335280048153944No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT38890.21300123616299826No Hit
GGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGT38530.21102950962613326No Hit
CCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGCCCGAAGCTTC37570.2057715721944933No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36660.20078748567075125No Hit
CGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTA35550.19470799551541754No Hit
GCCGAATCAACTAGCCCTGAAAATGGATGGCGCTAGAGCGTCGCACCTAT34970.1915313249838017No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA34670.18988821953641424No Hit
GTCACAATGTCTCCCTCGGATTTTCAAGGGACGACGGAGGTGCACCGGAC33040.18096067993894221No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG32890.18013912721524847No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA32270.17674337595731432No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGAT31860.17449779851255143No Hit
GATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCGCGA31740.1738405563335964No Hit
GGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGGCCGTCACCT31400.1719783701598906No Hit
GACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACCGAA29710.16272220947294108No Hit
CTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGCCCGA28950.15855967567289275No Hit
AAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCG27630.1513300117043878No Hit
GAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCC27210.1490296640780453No Hit
GCAGAATTCACCAAGCGTTGGCTTGTTCACCCACTAATAGGGAACGTGAG27000.14787949026487407No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG26870.14716747790433948No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT26720.14634592518064574No Hit
TCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCG25680.1406498262963691No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC25570.1400473542989937No Hit
AATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACCGAATCAA25140.13769223649107165No Hit
CTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCGC24680.13517280813841082No Hit
GCACGAAGGAGGTTCAACGGGTTACCCGACCCTTTCGGGCCAGGACAGAG24410.13369401323576208No Hit
GCGAACATGAGTGCCAGCTATCCTGAGGGAAACTTCAGAGGGAACCAGCT24240.13276292014890917No Hit
GCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCGCGAATGGCT23800.13035303215940752No Hit
GAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCTCGGGTTCTGCCTAGG23740.13002441106993004No Hit
ATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCTCGG23490.12865515653044046No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG23410.1282169950778038No Hit
GTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCT23060.12630003872251838No Hit
GGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGC22820.12498555436460838No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC22690.12427354200407381No Hit
GAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACGCAA22570.12361629982511882No Hit
CTACATTGACATGGGATGGATCTCGCTTCGACCGACACCCGAAATGAGCA22310.12219227510404966No Hit
TCTACATTGACATGGGATGGATCTCGCTTCGACCGACACCCGAAATGAGC22200.12158980310667425No Hit
GATGGATCTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGG21800.11939899584349092No Hit
GTCCAACTGCTGTTCACATGGAACCCTTCTCCACTTCAGTCTTCAAAGTT21560.11808451148558094No Hit
CCCATGTCCAACTGCTGTTCACATGGAACCCTTCTCCACTTCAGTCTTCA21210.11616755513029552No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTT21120.11567462349607928No Hit
GGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCA20710.11342904605131637No Hit
GTTGGCTCCTCGCGGGGTCGTGTGCGGTAACGCAAACGAACTCGGAGACG20680.11326473550657763No Hit
GTCGGATTCCCCTGGTCCGTGCCAGTTCTAAGTTGGCTGTTAGTCGCCGG20550.11255272314604306No Hit
GAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTC20170.1104714562460189No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCTTGCTTAAAACTCCT19920.10910220170652933No Hit
GTTTACCCGCGCTTGATTGAATTTCTTCACTTTGACATTCAGAGCACTGG19420.10636369262755016No Hit
ATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCGCGAA19420.10636369262755016No Hit
GGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGGCCGTCACCTG19380.10614461190123184No Hit
CGACTGTCTAATTAAAACAAAGCATTGCGATGGCCGTCACCTGGTGTTGA19310.10576122063017475No Hit
CTGCACTCGCGGCGGCTCCACCCGGGCTCACGCCCGAAGCTTCAAAGCAC19280.10559691008543602No Hit
GTCGATGGTACGTGCTATGCCTACCATGGTTGTAACGGGTAACGGGGAAT19180.10504920826964016No Hit
GTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTAC18970.10389903445646893No Hit
GTGAAGGCAGCCTCGGGTTCTGCCTAGGTGGGATCCTCGGTCCGTCGCGG18880.1034061028222527No Hit
AACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACGCAAACGAACTCG18800.10296794136961601No Hit
GCCGTTTACCCGCGCTTGATTGAATTTCTTCACTTTGACATTCAGAGCAC18570.10170822719328561No Hit
GTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCTGGCACGAAAGT18490.10127006574064895No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA6800.098.165331
TATTCCC8450.077.194986
TAATATT8650.077.0901263
ATATTCC8800.074.9483265
ATTCCCG8850.073.7079547
GCTATAT4150.055.90617145
TCTAGCC1206.002665E-1154.377487145
TTGTCTC6200.051.448332
TTAATAT13250.050.336412
AATATTC13400.049.2224964
CTTGTCT6800.046.9486351
ATTTGCT26550.046.4583321
TTGCTAC27000.046.173383
TGCTACT27950.044.6039854
GTCCAAC11500.041.0104681
CATGTCC8450.040.315143
AAGATTA14250.040.182713
AGATTAA14400.039.7641454
GTCGACT1105.238351E-639.5765381
TCATATG1553.4749974E-837.4169652
Produced by FastQC (version 0.11.5)