FastQCFastQC Report
Wed 2 Jan 2019
HC-E-1_S7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-E-1_S7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825811
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT99550.5452371576247487TruSeq Adapter, Index 2 (97% over 37bp)
ATTTGCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTC86930.47611718847131496No Hit
GCTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGC74510.40809262294947285No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT55240.30255048304561644No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG53380.29236322927181396No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC52850.2894604096480961No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATT52370.28683144093227614No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT50560.2769180380663716No Hit
CCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGCCCGAAGCTTC48650.26645693338467125No Hit
GTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTGCTGCCTT46380.25402410216610594No Hit
CGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTA44010.24104356913174477No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT42880.2348545386132519No Hit
GTTCGATTAGTCTTTCGCCCCTATGCTCAAGTTTGACGATCGATTTGCAC42770.23425206661587647No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT39980.2189711859551728No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCAC38250.20949594454190493No Hit
GGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGT37750.20675743546292577No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA36480.19980162240231877No Hit
CTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGCCCGA35400.1938864427917238No Hit
GCCGAATCAACTAGCCCTGAAAATGGATGGCGCTAGAGCGTCGCACCTAT35260.19311966024960964No Hit
GATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCGCGA33500.18348010829160302No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA33430.18309671702054592No Hit
GTCACAATGTCTCCCTCGGATTTTCAAGGGACGACGGAGGTGCACCGGAC33270.1822203941152726No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGAT33060.18107022030210135No Hit
GACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACCGAA31770.17400486687833516No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT31030.16995187344144602No Hit
AAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCG30690.1680896872677402No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG30440.16672043272825063No Hit
GCAGAATTCACCAAGCGTTGGCTTGTTCACCCACTAATAGGGAACGTGAG29500.16157203565976982No Hit
GAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCC28910.15834059494657443No Hit
GGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGGCCGTCACCT27990.1533017382412528No Hit
GGAAACTTCAGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC27980.1532469680596732No Hit
AATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACCGAATCAA27880.1526992662438774No Hit
TCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCG27560.15094662043333074No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG27080.14831765171751074No Hit
GAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCTCGGGTTCTGCCTAGG26610.14574345318327034No Hit
ATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCTCGG26240.14371695646482577No Hit
GCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCGCGAATGGCT26050.14267632301481367No Hit
GCGAACATGAGTGCCAGCTATCCTGAGGGAAACTTCAGAGGGAACCAGCT25070.13730884522001455No Hit
CCCATGTCCAACTGCTGTTCACATGGAACCCTTCTCCACTTCAGTCTTCA24860.13615867140684332No Hit
GGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGC24700.13528234850156998No Hit
GTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCAGCCT24350.1333653921462846No Hit
GCACGAAGGAGGTTCAACGGGTTACCCGACCCTTTCGGGCCAGGACAGAG24280.1329820008752275No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC24030.1316127463357379No Hit
TCTACATTGACATGGGATGGATCTCGCTTCGACCGACACCCGAAATGAGC24020.13155797615415835No Hit
CTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCGC23400.1281622248962242No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTT23400.1281622248962242No Hit
GAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACGCAA23100.12651911944883673No Hit
GTCGATGGTACGTGCTATGCCTACCATGGTTGTAACGGGTAACGGGGAAT21580.1181940518487401No Hit
GTTTACCCGCGCTTGATTGAATTTCTTCACTTTGACATTCAGAGCACTGG21540.11797497112242177No Hit
ATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACCGCGAA21310.11671525694609136No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG21300.11666048676451178No Hit
GAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTC21220.1162223253118751No Hit
GATGGATCTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGG21130.11572939367765886No Hit
GTCCAACTGCTGTTCACATGGAACCCTTCTCCACTTCAGTCTTCAAAGTT20900.11446967950132846No Hit
CCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACGC20770.11375766714079387No Hit
GTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTAC20670.11320996532499805No Hit
CGAAGCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTTTCGGC20570.1126622635092022No Hit
GTGAAGGCAGCCTCGGGTTCTGCCTAGGTGGGATCCTCGGTCCGTCGCGG20460.1120597915118268No Hit
CTGCACTCGCGGCGGCTCCACCCGGGCTCACGCCCGAAGCTTCAAAGCAC20320.11129300896971263No Hit
CCAATCCCTAGCACGAAGGAGGTTCAACGGGTTACCCGACCCTTTCGGGC20100.1100880649749618No Hit
CTACATTGACATGGGATGGATCTCGCTTCGACCGACACCCGAAATGAGCA20090.11003329479338222No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCTTGCTTAAAACTCCT19890.10893789116179058No Hit
CTACTACCACCAAGATCTGCACTCGCGGCGGCTCCACCCGGGCTCACGCC19860.10877358061705182No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG19640.107568636622301No Hit
GTTGGCTCCTCGCGGGGTCGTGTGCGGTAACGCAAACGAACTCGGAGACG19600.10734955589598266No Hit
GTCGGATTCCCCTGGTCCGTGCCAGTTCTAAGTTGGCTGTTAGTCGCCGG19580.10724001553282349No Hit
CGACTGTCTAATTAAAACAAAGCATTGCGATGGCCGTCACCTGGTGTTGA19580.10724001553282349No Hit
GGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGGCCGTCACCTG19300.10570645044859517No Hit
AACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACGCAAACGAACTCG18980.10395380463804851No Hit
GAACACTGTAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGGTGGGT18980.10395380463804851No Hit
GCAGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTCTGCCCT18620.10198207810118354No Hit
GGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCA18460.1011057551959102No Hit
GTCTTTCGCCCCTATGCTCAAGTTTGACGATCGATTTGCACGTCAGAACC18450.10105098501433062No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG18410.10083190428801228No Hit
CTTGATTCGGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGAT18350.10050328319853478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATA7000.0103.59361
TAATATT8400.087.191273
TATTCCC8700.082.5176546
ATATTCC8850.081.119055
ATTCCCG9050.079.313337
CTTGTCT7750.065.497891
TTGTCTC7600.064.880532
CTTCCGA1102.5465852E-1159.3308754
GTCTCAA9750.050.574934
ATTTGCT25850.050.4943661
AAGATTA16550.049.5121043
TTAATAT14900.049.153472
TTGCTAC26250.048.8961753
TGCTACT27300.047.0155534
AGATTAA17300.046.9464654
TCCGATC1402.728484E-1046.617126
AAAGATT17550.046.276442
CAAAGAT17500.045.9955561
CATGTCC8700.044.1761173
GGAAATT1851.8189894E-1243.1173361
Produced by FastQC (version 0.11.5)