FastQCFastQC Report
Wed 2 Jan 2019
HC-A-neg_S23_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-A-neg_S23_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51394
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA1640.3191033972837296No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG1620.31521189243880604No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT1430.27824259641203253No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1390.2704595867221855No Hit
GTCCAGTGGTGTGGATCCTAGGAGGACGGACTTGAAGTCATGTGCAGACA1050.20430400435848545No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTC970.1887379849787913No Hit
AAAGGAGCAGCGTCTCACATCACCTGGTCGGCTGATACTCCTGTGTGTCG850.1653889559092501No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG840.16344320348678834No Hit
AGCAACTCGTGGTGCAGTCGTTGCCAGATGGTGGGAAAGCAGAACAGTCC730.14203992683970892No Hit
GTCGAAGGTGTGATCGTTGTCAGTTGGATCAGGCAGGAAGCCAGCAACGT730.14203992683970892No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATCGGAC720.14009417441724714No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT720.14009417441724714No Hit
CTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG710.1381484219947854No Hit
TACAGCCTTGGAACTGGAGATGTTGACCGTGCCGTTTATTGGCACCTCAA710.1381484219947854No Hit
GAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTG680.13231116472740007No Hit
AGCAACGCCATCTGTGAACGTTTTGTTGAAGTTGTCCCAGATGCCCCAGC680.13231116472740007No Hit
CCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCAT670.1303654123049383No Hit
GTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTGCTGCCTT670.1303654123049383No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCAC630.12258240261509125No Hit
GGGACACGCTGCTAGAATCTTCCAGCGATACGCTGTTGAAACACTGGAGT620.1206366501926295No Hit
GTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATCGGACTCGGGGA620.1206366501926295No Hit
GGCAATACCGCGCATGATCTCCAAATCGCGATCAGAGTAGTTTCCACTCT610.11869089777016772No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATC600.11674514534770597No Hit
GACACAGTACTCGGCTGGAAGGTGTACAACAGTATCGGAACACCCAAACC590.11479939292524419No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT570.11090788808032065No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC570.11090788808032065No Hit
ATGACCCGGTTAGTTTTCCGAGTCAACTCTGGTGCAGACAGTGCCAACAG570.11090788808032065No Hit
GCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACC540.10507063081293536No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG520.10117912596801183No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAGC100.0070714243145.07
GAATATC151.2232736E-4145.02
TCGGCTA100.0070714243145.02
ATGTAGT100.0070714243145.09
TACAGCC202.11121E-6145.01
CAGACAC100.0070714243145.08
ATACCGC203.844976E-4108.755
CAGCCTT301.5849817E-596.6666643
AGCCTTG301.5849817E-596.6666644
TACCCAA259.335753E-487.0145
GGCAATA259.335753E-487.01
CAAAATC353.4062534E-582.857147
TGGAACT353.4062534E-582.857149
CTTGGAA353.4062534E-582.857147
ACCCAAA300.00192526572.59
AATACCG350.003547270862.1428574
TTGGAAC501.9922575E-458.08
ACAGCCT501.9922575E-458.02
CACACCT400.006018376454.3752
ATCCGAC553.1902822E-452.7272766
Produced by FastQC (version 0.11.5)