HC-A-neg_S23_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HC-A-neg_S23_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51394
Sequences flagged as poor quality	0
Sequence length	151
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT	182	0.3541269408880414	No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG	147	0.2860256061018796	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	138	0.2685138342997237	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	133	0.25878507218741487	No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA	109	0.2120870140483325	No Hit
GTGCAGTCGTTGCCAAATGGTGGGAAAGCAGAACAGTCCGACCGCAGTAC	107	0.20819550920340896	No Hit
CTGGAAGATTGCTCCTTCAAGGGCAATGTCCAGTGGTGTGGAACCTAGGA	86	0.16733470833171188	No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATC	79	0.1537144413744795	No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT	78	0.15176868895201776	No Hit
GGTGTCTCAAGAGACGGAACGCTCAGTCGAACACTCCAGCGACACAGCAC	75	0.14593143168463246	No Hit
AGCCGGGACAGGAACAGACGGATACGTTGTTGTTGATGCAACCATCGTCC	72	0.14009417441724714	No Hit
AGCAACGCCATCTGTGAACGTTTTGTTGAAGTTGTCCCAGATGCCCCAGC	70	0.1362026695723236	No Hit
GTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATCGGACTCGGGGA	69	0.13425691714986185	No Hit
TACAGCCTTGGAACTGGAGATGTTGACCGTGCCGTTTATTGGCACCTCAA	69	0.13425691714986185	No Hit
CTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC	67	0.1303654123049383	No Hit
GGGAATTCCTCGTTCATGGGGAGAATTACACACCCCAATCCCTAGCACGA	66	0.12841965988247656	No Hit
CACGTCAGAACCGCTGCGGACCTCCATCAGAGTCCAGAGGAGCGTGCGGG	64	0.12452815503755303	No Hit
CTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG	61	0.11869089777016772	No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT	59	0.11479939292524419	No Hit
TCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCG	59	0.11479939292524419	No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG	59	0.11479939292524419	No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTGCTATCGGAC	59	0.11479939292524419	No Hit
GTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTGCTGCCTT	59	0.11479939292524419	No Hit
GGAGATGTTGACCGTGCCGTTTATTGGCACCTCAAGAAGTTTGCTGGAAA	59	0.11479939292524419	No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTC	59	0.11479939292524419	No Hit
GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA	58	0.11285364050278243	No Hit
CCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCAT	58	0.11285364050278243	No Hit
TGTCATTTCTCCATCGCCCCGTGAGGTTGGGTACCACGGGTCACGTCAGT	58	0.11285364050278243	No Hit
GAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGTTTG	57	0.11090788808032065	No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC	56	0.1089621356578589	No Hit
GCAGAACTGGCGCTGTGGGATGAACCAAACGCTCGGTTAAGGTGCCTAAG	54	0.10507063081293536	No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC	53	0.1031248783904736	No Hit
CCACACCTGCAGGCTGGTTTCGCTGGGGCATCTGGGACAACTTCAACAAA	53	0.1031248783904736	No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA	52	0.10117912596801183	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	10	0.0070714243	145.0	145
AATCCCG	10	0.0070714243	145.0	5
AGCTTAC	10	0.0070714243	145.0	5
GACCCGG	15	1.2232736E-4	145.0	2
ACCCGGT	15	1.2232736E-4	145.0	3
CCCGGTT	15	1.2232736E-4	145.0	4
CGGTTAG	25	9.335753E-4	87.0	6
ACGCCAT	35	3.4062534E-5	82.85714	5
ATATACG	35	3.4062534E-5	82.85714	6
TATACGC	35	3.4062534E-5	82.85714	7
TAATATA	40	6.603221E-5	72.5	4
AATATAC	40	6.603221E-5	72.5	5
TACGCTA	40	6.603221E-5	72.5	9
CCGGTTA	30	0.001925265	72.5	5
TTAATAT	45	1.18314536E-4	64.44444	3
GCAACGC	45	1.18314536E-4	64.44444	2
CGTTTCG	45	1.18314536E-4	64.44444	3
TGACCCG	35	0.0035472708	62.142857	1
CGTCATT	35	0.0035472708	62.142857	145
TTTAATA	50	1.9922575E-4	58.0	2

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content