FastQCFastQC Report
Wed 2 Jan 2019
HC-A-3_S6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-A-3_S6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13015303
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA556120.4272816391596876No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA503800.38708280552515756No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA464260.35670318239997945No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT425220.32670772244026897No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT424990.32653100738415386No Hit
CCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCAT314020.2412698344402739No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG297970.22893819682876382No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTC284960.21894227126329677No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA276950.21278797735250574No Hit
GTACGCTCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGT273990.21051373141293753No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC260390.2000644933122187No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT258230.19840490843739864No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC257080.19752133315682316No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG252600.19407923119423343No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG245610.18870863014099631No Hit
CTCGGCTCGGGGGTCGATGAAGAGCGCAGCCAGCTGCGTGAATTAATGTG233030.17904308489783144No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG228910.17587758041437837No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT226550.17406433027337126No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA225000.1728734244604217No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGTACTCATTCCAATTACG201820.1550636201093436No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG196870.1512604047712143No Hit
CCCTTCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC196740.15116052234819274No Hit
GCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCAC192900.14821014923740156No Hit
GGTGAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAG189200.14536734181294128No Hit
GAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG185550.14256295070502778No Hit
GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATA178510.13715393333524392No Hit
ATCGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGT177220.13616279236833748No Hit
CTTAATCATTACCTCGTGTTCCGAAAACCAACAAAATAGAACCGAGGTCC175940.1351793346647404No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC175300.1346876058129419No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT173550.13334303473380527No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA171430.13171418291222264No Hit
GACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCGT171260.13158356743596364No Hit
GAAACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGCTTCGCCT169700.13038497835970472No Hit
GTAGATCTGGGCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCA168650.12957823571222274No Hit
GTTTATGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACT165890.12745765503884157No Hit
CGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCG165090.1268429939740934No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC164910.12670469523452507No Hit
TTCCTATCTCTTAGGACCGACTGACCCATGTCCAACTGCTGTTCACATGG160970.12367748949064036No Hit
GTTATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGC159590.12261719915394977No Hit
GCGAGACTACGCCCTGAACTTAAGCATATCACTAAGGGCAGGAAAAGAAA157000.12062723395682759No Hit
CTGGGAATTCCTCGTTCATGGGGAGAATTACACACCCCAATCCCTAGCAC156900.12055040132373407No Hit
CTCTAAGTGGTTTCACGTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCA154370.118606535706468No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG149150.11459587225898622No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCAC146900.112867138014382No Hit
CCCGGCCCGAACACTGTAAGGATTGACAGATTGAGAGCTCTTTCTTGATT143890.11055447575826702No Hit
GGCCTTTACTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGG142040.10913307204603688No Hit
AGGGAATCGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGC141360.10861061014100094No Hit
AGAAGTTACACCGACAGCAATAGATCGGTGAACTATTTAGTAGGTTAGAG141210.10849536119136066No Hit
GTTTCACGTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCT140810.10818803065898658No Hit
GTCCGTGCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGC137040.10529144039136086No Hit
CACGTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACG136930.10520692449495798No Hit
TGAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTT133510.10257924844315958No Hit
CTTTGAGCAGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTC133050.10222581833092936No Hit
CGGCATCGTTTATGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACC132160.10154200789639703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTCT40400.075.441761
CTAACAT138150.066.920991
GCTCGGG60400.065.646535
CCAGTAG56450.064.482922
CAGTAGT57850.063.152943
TAACATG146900.062.7870672
GGCGCCT13300.060.5640871
ACATGTG152650.060.4972574
TCTGCGT20200.059.9941561
AACATGT156850.058.9239773
CCACGTA20750.058.0542221
TGTGCGC160000.057.5826957
GCGCCAT127450.055.40091
TCGGGGG71800.055.1232267
CGGCATC42650.054.957551
CTGCGCG23000.053.9525681
AGGGAAT38850.053.4218441
CTGCGCC26900.052.6048971
AGTAGTC71600.051.530914
TGAGTAT20050.051.0327031
Produced by FastQC (version 0.11.5)