FastQCFastQC Report
Wed 2 Jan 2019
HC-A-3_S6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-A-3_S6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13015303
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA605170.4649680456920596No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA554480.4260215839769539No Hit
CTCGGCTCGGGGGTCGATGAAGAGCGCAGCCAGCTGCGTGAATTAATGTG509560.3915083651913444No Hit
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT456990.3511174499740805No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT447020.34345723645465653No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA416580.32006938294098874No Hit
CCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCAT332520.2554838715625752No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG307560.23630644634243242No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTC300080.23055936538703709No Hit
GTACGCTCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGT288670.2217927619510664No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT277560.21325665641437624No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC267740.20571169184459248No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC265370.20389075844027604No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA262410.20161651250070783No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG244870.18814006865610428No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG243510.1870951448460324No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT235960.18129428104747158No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA230330.17696860380430637No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG230190.17686103811797543No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGTACTCATTCCAATTACG220250.16922387438847947No Hit
CTTAATCATTACCTCGTGTTCCGAAAACCAACAAAATAGAACCGAGGTCC212420.1632078792172568No Hit
GAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG209590.16103351570071017No Hit
GCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCAC206420.15859792123164554No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG203020.15598561170646585No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC199610.1533656189179768No Hit
CCCTTCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC196730.1511528390848834No Hit
ATCGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGT190360.1462586003568261No Hit
GGTGAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAG186390.14320834482301334No Hit
GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATA182820.14046541982157465No Hit
GTTTATGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACT180710.13884425126330135No Hit
GACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCGT179560.13796067598272588No Hit
GAAACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGCTTCGCCT176940.13594766099567562No Hit
CTCTAAGTGGTTTCACGTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCA176650.13572484635970442No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT176580.13567106351653896No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA175140.13456467359999225No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC173430.13325083557409304No Hit
GTTATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGC166510.1279340173640214No Hit
GTAGATCTGGGCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCA165760.12735777261582001No Hit
AGAAGTTACACCGACAGCAATAGATCGGTGAACTATTTAGTAGGTTAGAG163240.12542159026186328No Hit
TGAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTT160910.12363138991078426No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG154680.11884471686905791No Hit
CACGTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACG154490.11869873486618021No Hit
GCGAGACTACGCCCTGAACTTAAGCATATCACTAAGGGCAGGAAAAGAAA152940.11750782905323065No Hit
CCCGGCCCGAACACTGTAAGGATTGACAGATTGAGAGCTCTTTCTTGATT148090.11378144634819488No Hit
AGGGAATCGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGC147880.1136200978186985No Hit
GTTTCACGTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCT146390.11247529158560504No Hit
CGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCG145570.11184526399423816No Hit
GTCCGTGCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGC142270.10930978710215197No Hit
CGGCATCGTTTATGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACC141040.10836474571510168No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG138510.1064208800978356No Hit
CTTTGAGCAGATCGCACGGGCTTGTCCCGGCGACGTGTCTTTCAAATGTC138350.10629794788488597No Hit
CTGAACTTAAGCATATCACTAAGGGCAGGAAAAGAAACTAACTAGGATTC138160.10615196588200829No Hit
GGCCTTTACTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGG137160.10538363955107308No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT135830.10436176553092924No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCAC131820.10128077694387906No Hit
CTTTACTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTT131450.10099649620143303No Hit
CTGGCTTCGGCCTCGGCTGCTCTGGACTGTCCTAGGAGTCGGGTTGTTTG131050.10068916566905894No Hit
TTCCTATCTCTTAGGACCGACTGACCCATGTCCAACTGCTGTTCACATGG130580.10032805229351942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTCT41550.080.624871
GCTCGGG98800.077.940045
TCGGGGG111050.069.789837
CCACGTA20300.067.866521
CTAACAT136650.065.585361
AGGGAAT36250.062.8086851
CCAGTAG54150.062.5330772
TAACATG145550.061.4245952
CAGTAGT55750.061.3889473
GGGGGTC126900.060.615699
CGGGGGT129000.060.078538
ACATGTG150800.059.430614
CGGCATC41600.058.9143941
AACATGT155750.057.8676873
CGGCTCG138450.056.666633
CTGCGCG19350.056.209321
TGTGCGC159600.055.691717
GCCGTAT8700.054.1741561
GGCTCGG146900.053.3083234
GCGCTAT9500.052.6651761
Produced by FastQC (version 0.11.5)